| siRNA抑制胃腺癌VEGF-C表达和淋巴管生成 |
| |
| 引用本文： | 邢春根,陈正荣,蒋银芬,吕孝东,吴永友,赵奎.siRNA抑制胃腺癌VEGF-C表达和淋巴管生成[J].肿瘤防治研究,2008,35(11). |
| |
| 作者姓名： | 邢春根 陈正荣 蒋银芬 吕孝东 吴永友 赵奎 |
| |
| 作者单位： | 苏州大学附属第二医院普外科,215007 |
| |
| 基金项目： | 苏州大学医学重点发展基金
|
| |
| 摘 要： | 目的 研究瘤内注射VEGF-C siRNA抑制胃癌移植瘤VEGF-C表达和肿瘤淋巴管生成的有效性.方法 建立裸鼠胃癌皮下移植瘤模型随机分为4组:VEGF-C sLRNA脂质体混合液组、单脂质体组、单siRNA组和PBS组,对肿瘤进行原位注射干预,记录各实验组肿瘤体积变化,免疫组织化学法检测各实验组肿瘤组织VEGF-C的表达,并通过抗人CD31和抗人LYVE-1单克隆抗体检测瘤体内血管和淋巴管生成情况.结果 免疫组化显示干扰组肿瘤细胞VEGF-C染色减弱,和对照组相比VEGF-C表达下调68.3%(P<0.01),而胃癌组织中淋巴管密度(LVD)值干预组和对照组分别为3.2±1.3、9.8±2.7,VEGF-C siRNA干预组LVD值下降至对照组的32.7%(P<0.01),而MVD值无明显差异(P>0.05).结论 瘤内注射VEGF-C siRNA能显著下调肿瘤组织内VEGF-C的表达,有效抑制肿瘤生长和瘤内淋巴管生成,而对肿瘤血管生成无影响.
|
| 关 键 词： | 胃腺癌 RNA干扰 血管内皮细胞生长因子-C 淋巴生成 |
Inhibition of Gastric Carcinoma Lymphangiogenesis with Small Interfering RNA Against VEGF-C |
| |
| XING Chun-gen,CHEN Zheng-rong,JIANG Yin-fen,LV Xiao-dong,WU Yong-you,ZHAO Kui.Inhibition of Gastric Carcinoma Lymphangiogenesis with Small Interfering RNA Against VEGF-C[J].Cancer Research on Prevention and Treatment,2008,35(11). |
| |
| Authors: | XING Chun-gen CHEN Zheng-rong JIANG Yin-fen LV Xiao-dong WU Yong-you ZHAO Kui |
| |
| Affiliation: | XING Chun-gen,CHEN Zheng-rong,JIANG Yin-fen,LV Xiao-dong,WU Yong-you,ZHAO Kui Department of General Surgery,The Second Affiliated Hospital,Soochow University,Suzhou 215004,China |
| |
| Abstract: | Objective To evaluate the inhibitory effects of VEGF-C siRNA on VEGF-C expression and tumor lymphangiogenesis in nude mice SGC7901 subcutaneous xenograft tumor models.Methods The nude mice model was divided randomly into 4 groups(n=6):VEGF-C siRNA-liposome-suspension,liposome only,siRNA only,and PBS injection.After injection intratumorally of every group the accumulation of tumor volume of each group was obtained.The expression of VEGF-C in tumor tissue was measured by Immunohistochemistry(IHC).And the stat... |
| |
| Keywords: | Gastric neoplasm RNA interference VEGF-C Lymphangiogenesis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
