| CTAB法抽提香榧种子胚乳DNA的改进 |
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| 引用本文： | 胡芳名,谭晓风,何德,李翠新.CTAB法抽提香榧种子胚乳DNA的改进[J].湖南林业科技,2002,29(1):1-5. |
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| 作者姓名： | 胡芳名 谭晓风 何德 李翠新 |
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| 作者单位： | 中南林学院经济林育种与栽培国家林业局重点实验室,湖南,株洲,412006 |
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| 基金项目： | 国家自然科学基金课题 (RAPD和AFLP分子标记构建香榧遗传图谱 )的部分内容,课题编号为 :39770 6 31 |
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| 摘 要： | 香榧种子胚乳DNA抽提是RAPD和AFLP分子标记构建遗传图谱的基础性工作。经多次实验，我们对CTAB法抽提香榧种子胚乳DNA进行了改进，即先将胚乳冷冻，抽提时加入β－巯基乙醇，抽提过程中用高盐TE重悬DNA－CTAB沉淀，最后用异丙醇沉淀DNA。OD260／OD280比值检测结果为平均1．824，而且DNA得率也较高，证明用此法抽提的DNA能完全满足RAPD和AFLP的分析的要求。
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| 关 键 词： | 种子 香榧 胚乳 CTAB DNA 抽提 萃取 |
Alteration in extraction of DNA from endosperm in Torreya grandis by CTAB method |
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| HU Fang-ming,TAN Xiao-feng,HE De,LI Cui-xin.Alteration in extraction of DNA from endosperm in Torreya grandis by CTAB method[J].Hunan Forestry Science & Technology,2002,29(1):1-5. |
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| Authors: | HU Fang-ming TAN Xiao-feng HE De LI Cui-xin |
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| Abstract: | It is a basic job in constructing the genetic graph of Torreya grandis to extract DNA from endosperm in its seeds.After several experimentations,some alteration was found in ertract DNA from endosperm in seeds by CTAB method:frozen endosperm in seeds in the beginning,added 2-ME in extracting DNA,suspended DNA-CTAB subsidence again by TE(higher salt)in extracting process,and subsided DNA by isopropanol in the end.The average was 1.824 in result of checking pureness of DNA.So the extracted DNA by this method can fulfil task of RAPD and AFLP analysis. |
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| Keywords: | Torreya grandis endosperm extraction of DNA CTAB |
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