禽流感H5N1型病毒对NIH小鼠的致病敏感性研究

目的观察禽流感H5N1型病毒对NIH小鼠的致病性。方法将NIH小鼠随机分为接毒组（20只）和对照组（10只），在负压感染实验室，接毒组于乙醚麻醉后给予AIVH5N1型病毒滴鼻，对照组予正常阴性尿囊液滴鼻，观察14d，记录小鼠的体温、体重、临床症状、死亡情况、病理变化、肺指数及抗体变化。结果NIH小鼠感染禽流感H5N1型病毒后第1天就出现精神不振，病程主要集中在第3—7天，临床症状主要表现为弓背、蜷缩、竖毛、颤抖、反应迟钝、活动减少、爱扎堆，体重（下降）出现减轻，体温降低，死亡率为40％；接毒组死亡小鼠肺指数为（2．21±0．40），较对照组的（0．44±0．23）明显增高，差异有统计学意义（P〈0．01）；肺组织病理严重改变，第8天才能检出抗体（OD值等于0．231）。结论禽流感H5N1型病毒对NIH小鼠有一定的致病性。

Pathogenicity of avian influenza H5N1 virus to NIH mouse

Objective To study the pathogenicity of avian influenza H5N1 virus to NIH mouse. Methods Anesthetized NIH mice were inoculated intranasally with avian influenza virus isolates at a biosafety laboratory. Throughout the 14-day experiment clinical signs were observed; body weight, body temperature, mortality and lung indexes were recorded. The blood was collected to analyze AIV antibodies. Results The H5N1 AIV could cause diseases in NIH mice between 3-7 days after the isolates were inoculated. The clinical signs, body weight, body temperature, mortality of NIH mouse had changed. The mortality of NIH mouse was 40%. Conclusion These reveal the H5N1 AIV was pathogenic in NIH mouse.