火绒草黄酮类成分的分离与结构鉴定

目的对火绒草的化学成分进行研究,为进一步开发利用该植物资源提供依据。方法采用反复正相硅胶、反相ODS、Sephadex LH-20等柱色谱以及高效液相色谱法等手段进行分离纯化,并通过理化性质与光谱分析鉴定化合物的结构。结果从火绒草体积分数为70%的乙醇溶液提取物中分离鉴定了9个黄酮类化合物,分别为槲皮素-3-O-β-D-吡喃葡萄糖苷(quercetin-3'-O-β-D-gluco-pyranoside,1)、槲皮素-3'-O-β-D-葡萄糖苷(quercetin-3'-O-β-D-glucopyranoside,2)、5,7,3',4'-tetra-hydroxy-3-methoxyflavone(3)、5,7,3',4'-tetrahydroxy-3-methoxyflavonol-3'-O-β-D-glucopyranoside(4)、山柰酚(kaempferol5,)、山柰酚-3-O-β-D-吡喃葡萄糖苷(kaempferol-3-O-β-D-glucopyranoside6,)、3-甲基-山柰酚(3-methyl-kaempferol,7)、3-甲醚-山柰黄素-7-O-β-D-吡喃葡萄糖苷(3-methylether-kaempferol-7-O-β-D-glucoside,8)、木犀草素-3'-O-β-D-葡萄糖苷(luteolin-3'-O-β-D-glucoside9,)。结论化合物2-57、8、为首次从火绒草属植物中分离得到。

Isolation and identification of Flavonoids of whole plant of Leontopodium leontopodioides(Willd.) Beauv.

Obejective To study the chemical constituents from the whole plant of Leontopodium leontopodioides (Willd.) Beauv.. Methods The compounds were isolated by chromatographic methods such as silica gel, ODS, Sephadex LH-20 and HPLC, and identified by the chemical and physical methods, especially spectral analysis. Results Nine flavonoids were isolated. The structures of them were identified as quercetin-3-O-b-D-glucopyranoside (1), quercetin-3'-O-β-D-glucopyranoside (2), 5,7,3',4'-tetrahydroxy-3-methoxyflavone (3), 5,7,3',4'-tetrahydroxy-3-methoxyflavonol-3'-O-β-D-glucopyranoside (4), kaempferol (5), kaempferol-3-O-β-D-glucopyranoside (6), 3-methylkaempferol (7), 3-methylether-kaempferol-7-O-β-D-glucoside (8), luteolin-3'-O-β-D-glucoside (9), respectively. Conclusions Compounds 2-5, 7 and 8 are obtained from Leontopodium genus for the first time.