阿司匹林-烟酰胺-锌络合物对胃癌细胞SGC-7901的生长抑制作用

目的：探讨阿司匹林-烟酰胺-锌络合物（商品名：佛立沙,WUY）对人胃癌细胞SGC-7901增殖的抑制作用。方法：用MTT法考察WUY对人胃癌细胞SGC-7901增殖的抑制作用,用流式细胞技术检测WUY对胃癌细胞周期的影响。结果：WUY在2、4、8、16mmol/L剂量时对胃癌SGC-7901细胞的增殖抑制率分别为9.95%、19.28%、37.36%、63.47%（P〈0.05）,平均IC50为11.06mmol/L。增殖抑制曲线表明,WUY对胃癌细胞有抑制作用且呈明显的量效和时效关系。流式细胞仪检测显示,WUY对胃癌细胞作用24h后,呈浓度依赖性改变细胞周期的分布,G0/G1期细胞比例下降,S期细胞比例和G2/M期细胞比例升高。结论：WUY能显著抑制人胃癌细胞SGC-7901的增殖,其作用可能与影响细胞周期有关。

Inhibitory effect of aspirin-niacinamide-zinc complex on human gastric carcinoma cell line SGC-7901

AIM:To investigate the inhibitory effeet of aspirin-niacinamide-zinc complex(Wuyisa,WUY)on human gastric carcinoma cell line SGC7901.METHODS:The inhibitory effect of WUY on SCC-7901 cells Was evaluated bv MTT analysis.The effect of WUY on SGC-7901 cell cycle was determined with flow cytometry.RESULTS:After treated with 2,4,8,16 mmol/L of WUY,the inhibition rates were 9.95%,19.28%,37.36%,63.47%(P＜0.05).The average IC50 of SGC-7901 cells was 11.06mmol/L.The dose effect relationship and time effect relationship were described in the growth curve.The cell cycle distribution Was changed in a concentrationdependent manner,after gastric cancer cell was treated for 24 hours with WUY by flow cytometry.Among phases of cell cycle,the G0/G1 phase ratio was decreased and the S and G0/M phase ratio were increased.CONCLUSION:WUY can obviously inhibit the growth of human gastric carcinoma cell line SGC7901 cells.It may be associated with the phases of cell cycle.