| 正交试验法优选甜菊叶绿原酸提取工艺 |
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| 引用本文： | 陈智,田景振. 正交试验法优选甜菊叶绿原酸提取工艺[J]. 山东中医药大学学报, 2020, 0(2): 193-196 |
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| 作者姓名： | 陈智 田景振 |
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| 作者单位： | ;1.山东中医药大学 |
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| 基金项目： | 山东省自然科学基金项目(编号:ZR2017LH070);山东省高等学校科技计划项目(编号:J17KA245)。 |
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| 摘 要： | 目的:以高效液相色谱法测定甜菊叶中绿原酸含量,正交试验法优选甜菊叶中绿原酸的最佳提取工艺。方法:以乙醇浓度、超声时间、料液比为考察因素,采用绿原酸提取率为指标,进行正交试验。结果:甜菊叶中绿原酸的最佳提取工艺为乙醇浓度60%,超声时间10 min,料液比1∶12,在最佳提取工艺下,绿原酸的提取率是4.03%。结论:通过正交试验优选的甜菊叶中绿原酸提取工艺稳定可行,适用于工业化生产。
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| 关 键 词： | 正交试验 甜菊叶 绿原酸 高效液相色谱法 乙醇浓度 超声时间 料液比 |
Optimization of Extraction Process of Chlorogenic Acid from Tianjuye(Stevia Rebaudiana) by Orthogonal Test |
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| CHEN Zhi,TIAN Jingzhen. Optimization of Extraction Process of Chlorogenic Acid from Tianjuye(Stevia Rebaudiana) by Orthogonal Test[J]. Journal of Shandong University of Traditional Chinese Medicine, 2020, 0(2): 193-196 |
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| Authors: | CHEN Zhi TIAN Jingzhen |
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| Affiliation: | ,Shandong University of Traditional Chinese Medicine |
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| Abstract: | Objective:To determine the content of chlorogenic acid from Tianjuye(Stevia Rebaudiana) by high performance liquid chromatography(HPLC) and optimize the extraction process of chlorogenic acid from Tianjuye by orthogonal test. Methods:Orthogonal test was carried out with ethanol concentration,ultrasonic time and material liquid ratio as factors,and chlorogenic acid extraction rate as index. Results:The optimum extraction technology of chlorogenic acid from Tianjuye was ethanol concentration of 60%,ultrasonic time of10 minutes,material liquid ratio of 1 ∶12. Under the optimum extraction technology,the extraction rate of chlorogenic acid was 4.03%. Conclusion:The extraction process of chlorogenic acid from Tianjuye optimized by orthogonal test is stable and feasible which is suitable for industrial production. |
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| Keywords: | orthogonal test Tianjuye(Stevia rebaudiana) chlorogenic acid high performance liquid chromatography ethanol concentration ultrasonic time material liquid ratio |
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