EGFR、Bax、Rb在高能电子线大鼠皮肤辐射损伤模型中的表达和意义

目的：探讨EGFR、Bax、Rb在高能电子线大鼠皮肤辐射损伤中的作用及机理，为临床治疗提供线索。方法：运用免疫化技术SP法检测EGFR、Bax、Rb在40例高能电子线大鼠皮肤辐射损伤模型中的表达，分析它们与照射剂量之间的关系。结果：（1）EGFR在40例照射的大鼠中，5、15、30、45Gy组阳性表达率分别为20％、40％、70％、100％；各照射剂组间有显著相关性（P＜0．05）。（2）Bax在40例照射的大鼠中，5、15、30、45Gy组阳性表达率分别为30％、30％、70％、70％；各照射剂量组同Bax蛋白阳性表达无相关性，但有趋势性（P＜0．05）。（3）Rb在40例照射的大鼠中5、15、30、45Gy组阳性表达率分别为30％、20％、40％、30％；各照射剂量组间Rb阳性表达无线性趋势性（P＞0．05）。（4）本实验对40例各照射剂量组实验对象研究发现Bax^ /Rb^ 共有9例，EGFR^ /Rb^ /Bax^ 共有5例，均无共同阳性趋势。结论：实验表明电离辐射能增加大鼠皮肤组织中EGFR、Bax、Rb蛋白的表达；EGFR、Bax蛋白的表达可能与照射剂量有关；EGFR能促进细胞的增殖与Bax,Rb蛋白促细胞凋亡共同作用下，参与3放射性皮肤损伤的形成。

Expression and significance of EGFR,Bax,Rb protein in a murine model of skin radiation injury

Objective\ The study was to find some valuable criteria for diagnosis and treatment of radiation injury of skin. Methods\ The expression of EGFR,Bax,Rb proteins was studied by SP immunohistochemistry in 40 mice using a skin radiation injury model.Their relationship to radiation dose was also investigated.Results (1) The expression rates of EGFR were 20%,40%,70%,and 100% in 5,15,30,and 45 Gy groups respectively.The expression of EGFR increased along with the increase of radiation dose,and there were significanty differences between groups ( P <0 05).(2) The expression rates of Bax were 30%,30%,70%,and 70% in 5,15,30,and 45 Gy groups,respectively,but there were no significant differences between these groups.(3) The expression rates of Rb were 30%,20%,40%,and 30% in 5,15,30,and 45 Gy groups,respectively,and there was no linear trend ( P >0 05).(4) There was no co\|expression tendency in 9 cases of Bax\ /Rb\ and 5 cases of EGFR\ /Rb\ /Bax\ among the 40 mice. Conclusion\ The experiment shows that radiation can increase the expression of EGFR,Bax,Rb proteins which might be related to poor healing of skin radiation injury.