乙型肝炎患者免疫学标志与其病毒DNA表达的相关性

目的 探讨乙型肝炎病毒感染的免疫学标志(HBVM)与乙型肝炎病毒脱氧核糖核酸(HBV DNA)的相关性.方法 采用酶联免疫吸附试验(ELISA)法和荧光定量聚合酶链反应(FQ-PCR)技术分别对1 573例乙型肝炎(下称乙肝)患者血清进行HBVM(HBsAg、抗-HBs、HBeAg、抗-HBe、抗-HBc)及HBV DNA检测.结果 HBsAg( )、HBeAg( )、抗-HBc( )模式,HBV DNA≥103 copy/ml检测率高达91.84%,其余HBsAg( )、抗-HBe( )、抗-HBc( )为58.13%,抗-HBe( )、抗-HBc( )模式为33.33%,抗-HBs( )、抗-HBe( )、抗-HBc( )为16.28%,抗-HBs( )模式为10.53%及HBVM全部阴性模式10.00%,并对HBVM各种模式HBV DNA表达的频率分布进行分析,结果显示HBsAg( )、HBeAg( )、抗-HBc( )模式,HBV DNA拷贝值由低(≥103 copy/ml)向高(108 copy/ml),且50.20%乙肝患者在107～108 copy/ml范围内.HBVM其余模式,多数患者集中在较低拷贝值范围内(103 copy/ml).分别是HBsAg( )、抗-HBe( )、抗-HBc( )为71.4%,抗-HBs( )、抗-HBe( )、抗-HBc( )为42.86%,抗-HBe( )、抗-HBc( )为66.70%.结论 血清HBVM阳性患者中72.38%血清HBV DNA≥103 copy/ml,且HBsAg( )、HBeAg( )、抗-HBc( )模式的血清中HBV DNA≥103 copy/ml检出率及HBV DNA的拷贝值均明显大于其他各种模式(P＜0.05),病毒复制活跃,传染力强,但其余模式仍可能存在病毒复制.

Correlation analysis of HBV DNA expression and serum HBV markers

Objective To investigate the relationship between HBV DNA expression and serum HBV markers.Methods FQ-PCR technique and ELISA method were applied to detect HBV DNA expression and serum HBV markers in 1 573 patients with hepatitis B.Results The amount of HBV DNA was different in 3 HBV immunological markers modes. The amount of HBV DNA expression was higher in HBV surface antigen, HBV e antigen and anti-HBcAg antibody positive mode than that of other modes, with 91.84% of positive rate. The positive rate of HBV DNA in other modes was 58.13% (HBV surface antigen, anti-HBeAg antibody and anti-HBcAg antibody positive mode), 33.33% (anti-HBeAg antibody and anti-HBcAg antibody positive mode), 16.28% (anti-HBsAg antibody, anti-HBeAg antibody and anti-HBcAg antibody positive mode), 10.53% (only anti-HBsAg antibody positive mode) and 10.00% (HBV markers negative mode), respectively.Conclusion Serum HBV DNA was above 103 copies/ml in 72.38% of HBVM positive patients, and serum HBV DNA positive rate and HBV DNA copies was highest in HBV surface antigen, HBV e antigen and anti-HBcAg antibody positive mode, with active viral replication and strong infective power. Viral replication might still exist in other modes.