表皮生长因子受体单克隆抗体对辐射诱导肺腺癌细胞SPC-A-1的增敏作用

目的:探讨表皮生长因子受体单克隆抗体(C225)作为靶向治疗药物联合放射治疗对肺腺癌细胞系(SPC-A-1)的增敏作用,为临床综合治疗非小细胞肺癌提供理论依据.方法:SPC-A-1细胞体外传代培养6代以上,取对数生长期细胞进行实验.SPC-A-1细胞分为对照组(PBS)、照射组(4 Gy)、C225组(100 nmol...

Radiosensitization of epithelial growth factor receptor monoclonal antibody on lung adenocarcinoma cell line SPC-A-1

Objective To explore the radiosensitivity of C225(cetuximab),an anti-epithelial growth factor receptor monoclonal antibody,which combined with irradiation against lung adenocarcinoma cell line SPC-A-1,and provide theoretical basis for clinical combined treatment in non-small lung cancer.Methods SPC-A-1 were cultivated in vitro for 6 passages,and the SPC-A-1 in logarithmic growth phase were seleted for experiment.The SPC-A-1 were divided into control group(PBS),irradiation group(4 Gy),C225 group(100 nmol·L-1) and irradiation+C225 group(4 Gy+100 nmol·L-1).The apoptosis of SPC-A-1 was observed by fluorescence microscope after Hoechst 33258 staining.SPC-A-1 were treated with different doses of 6-MV X-Rays including 0,2,4 and 8 Gy alone or together with C225(100 nmol·L-1),72 h after irradiation,the cells were divided into irradiation group and experimental group(irradiation+C22),the apototic rate was detected by flow cytometry(FCM).SPC-A-1 were divided into control group(PBS),C225 group(100 nmol·L-1),irradiation group(8 Gy) and irradiation+C225 group(8 Gy+100 nmol·L-1),48 h after irradiation,the cell cycle was determined by FCM.Results After staining by Hoechst 33258,the number of apoptotic cells in irradiation+C225 group was significantly higher than those in irradiation group and C225 group.In apoptosis experiment,the apoptotic rate in experimental group was higher than that in irradiation group(P<0.05).The cell cycle analysis showed that compared with control group,the number of cells in G0+G1 phase was increased in C225 group(P<0.05),the number of cells in G0+G1 and G2+M phrases in irradiation+C225 group was increased(P<0.05),the number of cells in S phrase of three groups was decreased(P<0.05).Conclusion The mechanism of radiosensitivity enhancement of C225 to SPC-A-1 may be related to arresting G0+G1 phases and inducing apoptosis.