| 番茄红素对心肌细胞缺氧损伤的作用机制 |
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| 引用本文： | 汪海宁,李吉林,简小莉,黄嵩,杨海燕.番茄红素对心肌细胞缺氧损伤的作用机制[J].中国当代医药,2013(30):7-10. |
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| 作者姓名： | 汪海宁 李吉林 简小莉 黄嵩 杨海燕 |
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| 作者单位： | 汕头大学医学院第一附属医院,广东汕头515041 |
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| 基金项目： | 国家自然科学基金（81300123） |
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| 摘 要： | 目的探讨番茄红素对心肌细胞沉默信息调节因子1（Sirtl）-自噬通路的作用。方法原代培养乳鼠心肌细胞予以无血清、无糖培养及缺氧干预，模拟心肌缺血，用番茄红素和Sirtl的抑制剂尼克酰胺干预。采用Westernblot法检测Sirtl、自噬相关基因6（Atg6）Beclinl及自噬相关基因8LC3蛋白的表达；RT—PCR技术检测心肌细胞Sirtl的mRNA表达。结果①在心肌缺氧中，番茄红素提高了细胞Sirtl蛋白和mRNA的表达水平；②番茄红素增加了心肌缺氧时自噬相关基因Beelinl和LC3蛋白的表达；③Sirtl的抑制剂尼克酰胺阻断了番茄红素对白噬蛋白的作用。结论番茄红素可能通过Sirtl激活自噬，从而减少心肌缺氧损伤。
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| 关 键 词： | 心肌细胞 缺氧 番茄红素 沉默信息调节因子1 尼克酰胺 自噬相关基因 |
The mechanism of Lycopene in neonatal rat cardiomyocytes under hypox- ic condition |
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| Affiliation: | WANG Hai-ning LI Ji-lin JIAN Xiao-li HUANG Song YANG Hal-yah The First Affiliated Hospital of Medical College of Shantou University in Guangdong Province, Shantou 515041, China |
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| Abstract: | Objective To investigate the effect of lycopene on Sirtl-Autophagy pathway in cardiomyocytes under hypoxic condition. Methods The primarily cultured neonatal rat cardiomyocytes were incubated under the condition of hypoxia in glucose free plus serum deprivation DMEM and exposed to lycopene or/and NAM. The protein level of Sirtl,Atg6 （Beclinl） and autophagic marker, LC3 were tested by Western blotting.The mRNA of Sirtl was detected by Real time PCR. Results Lycopene increased the protein and mRNA expression of Sirtl, and up regulated expression of Beclinl and LC3 protein under hypoxic condition. Blocking Sirtl by NAM could significantly decrease the effect of lycopene on autophagy in cardiomyocytes under hypoxic condition.Conclusion Lycopene may reduce myocardial damage by enhancing Sirtl-autophagy pathway under hypoxic condition. |
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| Keywords: | Cardiomyocyte Hypoxemia Lycopene（Lyc） Sirtl（silent information regulatorl） Niacinamide（NAM） Autophagy related gene（Atg） |
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