全反式维甲酸对兔颈动脉内皮损伤后内膜增殖和VEGF及VCAM-1表达的影响

目的 观察全反式维甲酸(atRA)对兔颈动脉内皮损伤后内膜增殖和血管内皮生长因子(VEGF)、血管细胞黏附分子-1(VCAM-1)表达的影响.方法 新西兰雄性大白兔36只,随机分为6组:治疗A、B组,对照A、B组,假手术A、B组.治疗组和对照组给予高脂饮食并行颈动脉内膜空气干燥术,假手术组手术暴露颈动脉而不损伤内膜,治疗组给予atRA连续灌胃.分别于术后7、28 d处死A、B组动物,取病变血管进行形态学观察.免疫组织化学方法检测病变血管VEGF、VCAM-1表达水平.结果 假手术组动脉内膜光滑,无粥样硬化病变及VCAM-1表达.对照组术后7 d动脉内膜层出现泡沫细胞,平滑肌细胞增殖,VEGF、VCAM-1表达明显增加,28 d时血管内膜明显增厚,有粥样斑块形成.治疗组动脉内膜增生较轻,28 d时内膜面积低于对照组(t=4.770,P<0.001),动脉粥样硬化病变轻于对照组(H=4.89,P<0.05),VEGF、VCAM-1表达低于对照组(t=3.280～4.288,P<0.01、0.05).结论 atRA可抑制兔颈动脉内皮损伤后新生内膜增殖,下调VEGF、VCAM-1表达是其作用机制之一.

EFFECTS OF ALL-TRANS RETINOIC ACID ON INTIMA PROLIFERATION AND VEGF AND VCAM-1 EXPRESSIONS OF RABBIT CAROTID ARTERY AFTER ITS ENDOTHELIUM INJURY

Objective To observe the effects of all-trans retinoic acid(atRA) on intimal proliferation,the expressions of vascular endothelial growth factor(VEGF) and vascular cell adhesion molecule-1(VCAM-1) after carotid endothelium injury in rabbits.Methods Methods Thirty-six New Zealand rabbits were randomized to treatment groups(A,B),control groups(A,B) and sham-operation groups(A,B).The rabbits in treatment groups and control groups were fed with high cholesterol chow and subjected to carotid endothelial injury b...