| UHPLC-Q-Orbitrap HRMS法分析精制冠心软胶囊化学成分 |
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| 引用本文： | 杨杰,李卓伦,孟海阳,左莉华,周霖,杨亚鹏,张爱玲,韩超,孙志,张晓坚.UHPLC-Q-Orbitrap HRMS法分析精制冠心软胶囊化学成分[J].中草药,2020,51(13):3406-3415. |
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| 作者姓名： | 杨杰 李卓伦 孟海阳 左莉华 周霖 杨亚鹏 张爱玲 韩超 孙志 张晓坚 |
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| 作者单位： | 郑州大学第一附属医院 药学部, 河南 郑州 450052;河南省精准临床药学重点实验室, 河南 郑州 450052;郑州大学第一附属医院 临床药学科, 河南 郑州 450052;河南省精准医学临床质谱工程研究中心, 河南 郑州 450052;郑州大学第一附属医院 药学部, 河南 郑州 450052;河南省精准临床药学重点实验室, 河南 郑州 450052;河南省精准医学临床质谱工程研究中心, 河南 郑州 450052;郑州市临床质谱重点实验室, 河南 郑州 450052;郑州人民医院 药学部, 河南 郑州 450052;郑州大学第一附属医院 药学部, 河南 郑州 450052;郑州大学第一附属医院 临床药学科, 河南 郑州 450052;河南省精准医学临床质谱工程研究中心, 河南 郑州 450052 |
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| 基金项目： | 国家重点研发计划（2017YFC0909900）；郑州大学第一附属医院院内基金（2015） |
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| 摘 要： | 目的建立一种对中药制剂精制冠心软胶囊中复杂化学成分快速、高效的鉴定分析方法,为系统研究其化学组成提供基础数据。方法应用超高效液相色谱-四级杆/静电场轨道阱高分辨质谱(UHPLC-Q-Orbitrap HRMS)技术对精制冠心软胶囊中的化学成分进行鉴定分析。通过多级离子碎片信息与对照品比对,结合文献数据进行准确定性。结果共鉴定出精制冠心软胶囊中43种化学成分,主要包括黄酮类、苯酞类、有机酸类、醌类及其他等。结论本方法可系统、准确、高效鉴定精制冠心软胶囊中化学组成,为其物质基础和质量控制研究提供科学理论依据。
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| 关 键 词： | 精制冠心软胶囊 超高效液相色谱-四级杆/静电场轨道阱高分辨质谱 黄酮类 酚酞类 有机酸类 醌类 |
| 收稿时间： | 2019/11/7 0:00:00 |
Constituent analysis of Jingzhi Guanxin Soft Capsule based on UHPLC-Q-Orbitrap HRMS |
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| YANG Jie,LI Zhuo-lun,MENG Hai-yang,ZUO Li-hu,Zhou Lin,YANG Ya-peng,ZHANG Ai-ling,HAN Chao,SUN Zhi,ZHANG Xiao-jian.Constituent analysis of Jingzhi Guanxin Soft Capsule based on UHPLC-Q-Orbitrap HRMS[J].Chinese Traditional and Herbal Drugs,2020,51(13):3406-3415. |
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| Authors: | YANG Jie LI Zhuo-lun MENG Hai-yang ZUO Li-hu Zhou Lin YANG Ya-peng ZHANG Ai-ling HAN Chao SUN Zhi ZHANG Xiao-jian |
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| Affiliation: | Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China;Henan Key Laboratory of Precision Clinical Pharmacy, Zhengzhou 450052, China;Department of Clinical Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China;Henan Engineering Research Center of Clinical Mass Spectrometry for Precision Medicine, Zhengzhou 450052, China;Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China;Henan Key Laboratory of Precision Clinical Pharmacy, Zhengzhou 450052, China;Henan Engineering Research Center of Clinical Mass Spectrometry for Precision Medicine, Zhengzhou 450052, China;Zhengzhou Key Laboratory of Clinical Mass Spectrometry, Zhengzhou 450052, China;Department of Pharmacy, People''s Hospital of Zhengzhou, Zhengzhou 450052, China;Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China;Department of Clinical Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China;Henan Engineering Research Center of Clinical Mass Spectrometry for Precision Medicine, Zhengzhou 450052, China |
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| Abstract: | Objective In order to establish a rapid and efficient analysis method for identification of the complex components in Jingzhi Guanxin Soft Capsule, and provide the basic research data for the systematic elaboration of its chemical constituents. Methods An ultra-high performance liquid chromatography-quadrupole/Orbitrap high resolution mass spectrometry (UHPLC-Q-Orbitrap HRMS) was used for the identification analysis of the components in Jingzhi Guanxin Soft Capsule, and the multistage fragments ions data was compared with the standard substance and literature consulting. Results Forty-three compounds were identified in this study, including flavones, phthalides, organic acids, quinones and other categories. Conclusion The chemical constituents of Jingzhi Guanxin Soft Capsule are identified systematically, accurately and efficiently, which provide the theory basis for the further research of its pharmacodynamic material basis and quality control. |
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