百合组培中鳞片处理及其颜色变化与鳞茎形成的关系

 研究了百合鳞片发育程度、切割段数、形态及生理变化、培养基的P 和K含量与鳞茎形成的关系。结果表明: 取中层鳞片、每片横切6 段的繁殖系数最高, 基部鳞片结鳞茎数最多, 且鳞茎发生最早。鳞片由基部向顶部繁殖系数递减。培养过程中鳞片颜色和形态变化依次为乳白色、浅黄、浅紫色、绿紫、鳞片干枯、鳞茎增大, 鳞片由浅黄变为绿紫色过程中叶绿素含量增加, 淀粉和可溶性糖含量下降。适当增加培养基中P、K的含量可提高鳞茎形成的数量和质量。

Studies on the Relation between Scale Treatment,Color Change and Lily Bullet Formation in Tissue Culture

The relations were studied between lily bullet formation and physiological age of scale , cutting ways , phosphorus (P) and potassium (K) concentrations in medium , anatomy and physiological changes. The results showed that middle layer scale crosscut into 6 sections had the highest propagation coefficient . Compared with others sections , the scale base had the highest propagation coefficient and the earliest generation of bullet , propagation coefficient decreased from the base to top of scale. Scale color and shape changed distinctly along with milky white →pale yellow →pale purple →green purple →scale withering and bullet growing. Chlorophyll content of scale increased , but starch and sugar contents of scale decreased. The suitable increases of phosphorus and potassium concentrations in medium increased the quantity and quality of bullet .