| 缓释骨形态发生蛋白-2听泡内植入对豚鼠内耳功能的影响 |
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| 引用本文： | 李雪盛,孙建军,姜伟,刘肖.缓释骨形态发生蛋白-2听泡内植入对豚鼠内耳功能的影响[J].听力学及言语疾病杂志,2010,18(1):39-42. |
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| 作者姓名： | 李雪盛 孙建军 姜伟 刘肖 |
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| 作者单位： | 1. 海军总医院全军耳鼻咽喉科中心,北京,100048
2. 海军总医院病理科,北京,100048 |
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| 基金项目： | 中国博士后科学基金项目 |
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| 摘 要： | 目的观察缓释骨形态发生蛋白-2（bone morphogenetic protein-2,BMP-2）植入豚鼠听泡内的成骨诱导情况及对耳蜗功能的影响。方法制备猪小柱状脱细胞松质骨,与BMP-2复合。选择20只豚鼠,耳后进路进入听泡,左耳植入复合BMP-2的脱细胞松质骨,右耳植入单纯脱细胞松质骨作为对照。分别在植入前、植入即刻、植入后3个月对动物进行ABR测试。并在植入3个月后行组织学及耳蜗基底膜铺片硝酸银染色观察,了解BMP-2诱导成骨情况及对听泡、耳蜗大体结构和毛细胞形态的影响。结果术后动物健康状况良好。植入后即刻,实验耳及对照耳ABR反应阈略有下降,3个月时恢复正常。植入3个月后,耳蜗及听泡大体形态正常,组织学检查可见含BMP-2脱细胞松质骨孔隙内有新骨形成,未见耳蜗及听泡骨质异常增生,镫骨底板关节无固定;硝酸银染色示毛细胞形态正常,无毛细胞缺失。结论缓释BMP-2听泡内植入可诱导新生骨形成,但对动物听泡大体形态无影响,不会引起局部骨质异常增生,对动物听功能无影响,有可能应用于听骨链缺损修复重建。
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| 关 键 词： | 听骨赝复物 骨形态发生蛋白 耳蜗 反应阈 |
The Affection on Cochlea Function of Guinea-pig by Transplanting Controlled Release BMP-2 into the Middle Ear |
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| Li Xuesheng,Sun Jianjun,Jiang Wei,Liu Xiao.The Affection on Cochlea Function of Guinea-pig by Transplanting Controlled Release BMP-2 into the Middle Ear[J].Journal of Audiology and Speech Pathology,2010,18(1):39-42. |
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| Authors: | Li Xuesheng Sun Jianjun Jiang Wei Liu Xiao |
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| Affiliation: | Li Xuesheng,Sun Jianjun,Jiang Wei,Liu Xiao(Center of Otolaryngology of PLA,Navy General Hospital,Beijin,100037,China) |
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| Abstract: | Objective The controlled release BMP-2 and implantation into the acoustic bulla of the guinea-pig and the effects on the cochlea were observed.Methods The acellular cancellous bone was prepared ,accompanied with BMP-2. The material accompanied with BMP-2 was implanted into one acoustic bulla of the animal,and the opposite side of the acoustic bulla was implanted with acellular cancellous bone without BMP-2. A total of 20 guinea-pigs were undergone this procedure. The ABR was tested on the animals before and... |
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| Keywords: | Prothesis Bone morphogenetic protein Cochlea Hearing threthold |
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