益气活血片对脑缺血神经细胞凋亡及相关基因表达的影响

目的]研究益气活血片(主要由黄芪、川苛、当归、石菖蒲、全竭、益毋草、冰片等组成)对不完全性脑缺血后大鼠海马和额叶皮层细胞凋亡及相关基因blc—2表达的影响。方法]建立不完全性脑缺血大鼠模型，治疗组在建立模型后每天灌服益气活血片至实验结束。应用流式细胞仪测定细胞群中细胞凋亡的比例和blc—2蛋白的含量，并结合常规病理观察组织学改变。结果]模型组大鼠组织学可见早期凋亡的形态学改变，并且海马、额叶神经细胞凋亡以及blc—2蛋白含量较假手术组升高；益气活血片治疗后凋亡细胞减少，但海马区仍高于假手术组，blc—2蛋白水平有所下调，接近假手术组。结论]不完全性脑缺血可引起神经细胞凋亡，益气活血片对此有一定的治疗作用。

Effects of Yiqi Huoxue Tablet on Nerve Cell Apoptosis and Expression of Related Gene in Rats with Cerebral Ischemia

Objective] To observe the apoptosis of cortical cells and expression of related gene bcl-2 in hippocampal gyrus and frontal lobe of rats with incomplete cerebral ischemia (ICI) and to investigate the effects of Yiqi Huoxue Tablet (YHT, mainly composed of Radix Astragali , Rhizoma Chuanxiong, Radix Angelicae Sinensis, Herba Leonuri, Rhizoma Acori Tatarinowii, Scorpio, etc) . Methods ] Rats were randomized into six groups: model group, mimic operation group, nimodipine group and low-dose, moderate-dose and high-dose YHT groups. Flow cytometry was used to detect the apoptotic rate and bcl-2 protein content in cortical cells. Histological changes were also observed under light microscope. Results] Early apoptotic changes were found in the model group and the apoptotic rate and bcl-2 protein content were higher than those in the mimic operation group; after treatment of YHT, the apoptotic rate in frontal lobe was decreased as compared with that in the model group and that in hippocampal gyrus remained higher than that in the mimic operation group and bcl-2 protein content was reduced to the level of the mimic operation group. Conclusion ] ICI is related to the apoptosis of nerve cells and YHT has been proved an effectual drug for the apoptosis.