机械牵张应变对人牙周膜细胞内游离钙离子浓度的影响

目的:观察机械牵张应变作用下,人牙周膜细胞(HPDLCs)内游离Ca2 浓度的变化。方法:体外培养HPDLCs,利用动态机械应变细胞加载装置,对1%、10%和20%应变组的细胞分别进行30、60和120min的动态牵张加载,通过流式细胞仪和激光共聚焦显微镜检测细胞内游离Ca2 浓度的变化,并应用SPSS13.0软件包对数据进行方差分析。结果:当HPDLCs加载30min时,各组检测到的胞内游离Ca2 浓度与对照组无显著差异(P>0.05);60min时,各个应变组的胞内Ca2 浓度明显升高,与对照组相比均有非常显著的差异(P<0.01);加载时间延长到120min时,各组Ca2 浓度又降至对照组水平(P>0.05)。结论:机械牵张应变可通过钙离子信号系统影响HPDLCs的生物学活性。

Effects of mechanical stretching on intercellular free calcium concentration of human periodontal ligament cells

PURPOSE:The purpose of this study is to investigate the effects of mechanical stretching on the concentration of free calcium in the human periodontal ligament cells(HPDLCs).METHODS:HPDLCs were cultured in vitro,and cells which were divided into strain 1%,10% and 20% group were stretched by dynamic mechanical strain unit for 30,60 and 120 minutes,respectively.Free calcium concentration was examined through flow cytometry and laser scanning confocal microscope.The data was analyzed by one-way ANOVA using SPSS13.0 software package.RESULTS:The results showed that there was no significant difference of the concentration of the free Ca2 in cells between each group after 30 minutes of loading(P>0.05),which was significantly higher than that in the controls by loading stretch for 60 minutes(P<0.01).As the application time of stretch was prolonged,the concentration of free calcium decreased to the level of controls after 120 minutes(P>0.05).CONCLUSION:The free calcium may be a mechanosensor which transduces the mechanical stretching into HPDLCs and regulates the physiology of cells.Supported by Natiural Science Foundation of Shanghai Municipality(Grant No.07ZR14070)and Shanghai Leading Academic Discipline Project(Grant No.T0202).