|
|||||
|
|
| 氧化槐果碱在Caco-2细胞模型中的吸收机制研究 | |
| 引用本文： | 冯志强,谢智勇,廖琼峰,谭晓婧,姚美村,张蕾.氧化槐果碱在Caco-2细胞模型中的吸收机制研究[J].中国中药杂志,2011,36(17):2399-2403. |
| 作者姓名： | 冯志强 谢智勇 廖琼峰 谭晓婧 姚美村 张蕾 |
| 作者单位： | 1. 中山大学药学院,广东广州510006;广州中医药大学中药学院,广东广州510006 2. 中山大学药学院,广东广州,510006 3. 广州中医药大学中药学院,广东广州,510006 |
| 基金项目： | 国家自然科学基金项目(30901953);国家教育部新教师基金项目(20094425120001);国家"重大新药创制"科技重大专项(2009ZX09304-003,2009ZX09501-017) |
| 摘 要： | 目的:研究氧化槐果碱在Caco-2细胞模型中的吸收机制.方法:MTT实验考察氧化槐果碱在Caco-2细胞中的安全浓度范围,再利用Caco-2细胞单层模型研究氧化槐果碱的双向转运机制,以转运量及表观渗透系数(Papp)为观测指标,考察时间、浓度、pH和P-gp抑制剂维拉帕米对其吸收的影响.结果:氧化槐果碱在Caco-2细胞模型中的转运与时间和浓度呈正相关,并受pH影响;P-gp抑制剂维拉帕米对其转运无影响,从单层细胞层顶端(AP)到基底端(BL)的转运与基底端到顶端的转运大致相同.结论:氧化槐果碱在Caco-2细胞模型中的吸收是被动转运. |
| 关 键 词： | 氧化槐果碱 Caco-2细胞模型 HPLC 被动转运 |
| 收稿时间： | 2010/10/19 0:00:00 |
Absorption mechanism of oxysophocarpine across Caco-2 cell monolayer mode |
|
| FENG Zhiqiang,XIE Zhiyong,LIAO Qiongfeng,TAN Xiaojing,YAO Meicun and ZHANG Lei.Absorption mechanism of oxysophocarpine across Caco-2 cell monolayer mode[J].China Journal of Chinese Materia Medica,2011,36(17):2399-2403. | |
| Authors: | FENG Zhiqiang XIE Zhiyong LIAO Qiongfeng TAN Xiaojing YAO Meicun and ZHANG Lei |
| Affiliation: | School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China;Guangzhou University of Chinese Medicine, Guangzhou 510006, China;School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China;Guangzhou University of Chinese Medicine, Guangzhou 510006, China;Guangzhou University of Chinese Medicine, Guangzhou 510006, China;School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China;Guangzhou University of Chinese Medicine, Guangzhou 510006, China |
| Abstract: | Objective : To investigate the absorption mechanism of oxysophocarpine across Caco-2 cell monolayer model. Method : The safety concentration of oxysophocarpine in Caco-2 cell was first selected by using MTT method. Then the Caco-2 cell monolayers drug transport model was assigned to study the bi-direction transport mechanism of oxysophocarpine by evaluating the influent factors such as time, concentration, pH, P-gp inhibitor of verapamil, on its absorption characterization. Result : In the Caco-2 cell monolayer model, the transport volume was correlated positively with the time and concentration of oxysophocarpine, and affected by pH value. Verapamil had no influence on its transport since the transport of oxysophocarpine from apical (AP) to basolateral (BL) was similar to the transport from basolateral to apical. Conclusion : The intestinal absorption mechanism of oxysophocarpine was deduced as passive transference by Caco-2 cell monolayer model. |
| Keywords: | oxysophocarpine Caco-2 cell monolayer model HPLC passive transference |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ | |
