Genotyping by RAPD-PCR analyses of Malassezia furfur strains from pityriasis versicolor and seborrhoeic dermatitis patients |
| |
Authors: | Rinaldo F Gandra Rita C G Simão Flávia E Matsumoto Bosco C M da Silva Luciana S Ruiz Eriques G da Silva Walderez Gambale Claudete R Paula |
| |
Affiliation: | Centro de Ciências Médicas e Farmacêuticas, Universidade Estadual do Oeste do Paraná, 2069, Cascavel, 85814-110, PR, Brazil. rfgandra@unioeste.br |
| |
Abstract: | Malassezia furfur is lypophilic yeast commonly associate with dermatological disorders. In the present work, we described the isolation of
47 M. furfur strains from three groups of patients: pityriasis versicolor (21 isolates), seborrhoeic dermatitis (15 isolates) and seborrhoeic
dermatitis of the HIV positive patients (11 isolates). To investigate the identity of the strains at molecular level, DNA
genomic of M. furfur strains were prepared and used to RAPD-PCR analyses. RAPD assay were carried out using two decamer primers and bands pattern
generated were analyzed by an Unweighted Pair-Group Method (UPGMA). Dendrogram established a distinct differentiation between
M. furfur isolates from pityriasis versicolor and seborrhoeic dermatitis patients with or without AIDS. We concluded that RAPD typing
presented a high discriminatory power between strains studied in this work and can be applied in epidemiological investigation
of skin disease causing by M. furfur. |
| |
Keywords: | AIDS Malassezia furfur pityriasis versicolor RAPD-PCR seborrhoeic dermatitis |
本文献已被 PubMed SpringerLink 等数据库收录! |