时间分辨荧光免疫技术检测HBV血清标志物临床应用评价

目的 对时间分辨荧光免疫分析技术（TRFIA）检测乙型肝炎病毒（HBV）血清标志物进行临床应用评价。方法 用TRFIA、微粒子酶免疫分析（MEIA）和酶联免疫吸附试验（ELISA）同时检测86例HBV感染者和50例正常人血清的HBsAg、抗-HBs、HBeAg、抗-HBe和抗-HBc，对三种方法的检测结果进行比较分析。结果 TRFIA法同MEIA法比较，五项标志物的Kappa值分别为1.000、0.847、0.934、0.698、0．877，均显示二者之间结果的高度一致性；TRFIA法同ELISA法比较，五项标志物的阳性检出数前者均高于后者。结论 TRFIA技术检测HBV血清标志物灵敏度高、特异性强，可替代MEIA和ELISA而成为一种常规检测方法。

The application of time-resolved fluorescence immunoassay for detection of HBV markers in serum

Objective To estimate the clinical application of time-resolved fluorescence immunoassay(TRFIA) in detecting HBV markers in serum.Methods HBsAg,anti-HBs,HBeAg,anti-HBe and anti-HBc in serum in 86 patients with hepatitis B virus infection and 50 healthy persons were detected by TRFIA,microparticle enzyme immunoassay(MEIA)and enzyme linked immunosorbent assay(ELISA),simultaneously.The results of these three kinds of methods were compared and analyzed.Results The method of TRFIA was compared with MEIA,and the kappa were 1.000,0.847,0.934,0.698 and 0.877,respectively.All of them showed there was a powerful conformability between the results of the two methods.Compared with ELISA,the positive cases of HBV markers of the method of TRFIA was more.Conclusion To detect HBV markers in serum,TRFIA offers many benefits in sensitivity,specificity,accuracy and practicability,and may be a routine method instead of MEIA and ELISA.