| 番木瓜环斑病毒单克隆抗体的制备及在病毒分型上的初步应用 |
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| 引用本文： | 林壁润,李英杰,欧阳明辉,范怀忠.番木瓜环斑病毒单克隆抗体的制备及在病毒分型上的初步应用[J].Virologica Sinica,1989(2). |
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| 作者姓名： | 林壁润 李英杰 欧阳明辉 范怀忠 |
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| 作者单位： | 华南农业大学植保系 广州(林壁润，范怀忠)，第一军医大学疟疾免疫研究室 广州(李英杰，欧阳明辉) |
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| 摘 要： | 本试验是用番木瓜环斑病毒(Papaya ringspot virus, PRV)提纯制剂免疫的BALB/c小白鼠脾细胞与Sp~2/o-Ag14骨髓瘤细胞融合,获得三个能稳定传代并分泌抗番木瓜环斑病毒的单克隆抗体的杂交瘤细胞系。其中23H1 McAb的效价较高,用ELISA检测,腹水抗体效价高达1:76800,能被PRV兔抗血清所阻断。这3个杂交瘤细胞系产生的单抗与TMV和CMV无血清交叉反应。它们可把PRV四个毒株初步区分为三个血清型。
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| 关 键 词： | 番木瓜环斑病毒 单克隆抗体 血清型 |
Preparation of Monoclonal Antibodies to Papaya Ringspot Virus and Application in Serotyping |
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| Lin Bi-run Li Ying-jie Ouyang Min-hui Faan Hwei-chung . South China Agricultural University,Guangzhou..Preparation of Monoclonal Antibodies to Papaya Ringspot Virus and Application in Serotyping[J].中国病毒学(英文版),1989(2). |
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| Authors: | Lin Bi-run Li Ying-jie Ouyang Min-hui Faan Hwei-chung South China Agricultural University Guangzhou |
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| Affiliation: | Lin Bi-run~2 Li Ying-jie~3 Ouyang Min-hui~3 Faan Hwei-chung~2 2. South China Agricultural University,Guangzhou.
3. The Frist Medicine University of PLA,Guangzhou. |
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| Abstract: | Three stable hybridoma cell lines secreting monoclonal antibodies against papaya ringspot virus were produced by fusing spleen cells of mice immunized with papaya ringspot virus(PRV-1) to mouse myeloma cell lines Sp2/0-Ag14. of three hybridoma cell lines, the titer of 23H1 secreting monoclonal antibody was found to be the highest, when testing by ELISA method, the titer of 23H1 inascitie fluid was 1:76800. Three cell lines secreting monoclonal antibodies were specific for papaya ringspot virus, not reactive to CMV and TMV. McAbs 23H1 and 22A12-B2 were reactive to isolates PRV-1, PRV-2 and PRV-15, and 22A12-E12 was reactive to isolates PRV-1 and PRV-2, Three serotypes of PRV were proposed by the authors after testing these monoclonal antibodies with four isolates of PRV. |
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| Keywords: | Papaya ringspot virus Monoclonal antibody Serotype |
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