不同生境人参茎叶总皂苷对心律失常小鼠的作用比较

目的 探讨园参茎叶总皂苷(GSLS)和林下山参茎叶总皂苷(MSLS)对心律失常小鼠的改善作用.方法 将80只SPF级BALB/c小鼠随机分为正常组、模型组、园参茎叶总皂苷低(GSLS-L)、中(GSLS-M)、高(GSLS-H)剂量组、林下山参茎叶总皂苷低(MSLS-L)、中(MSLS-M)、高(MSLS-H)剂量组....

Comparative study on the effects of ginseng stem-leaf saponins in different habitats on mice with arrhythmia

Objective To explore the efficacy of garden ginseng stem-leaf saponins (GSLS) and mountain cultivated ginseng stem-leaf saponins (MSLS) on mice with arrhythmia. Methods Eighty SPF BALB/c mice were randomly divided into normal group, model group, GSLS low (GSLS-L), medium (GSLS-M), high (GSLS-H) dose groups, MSLS low (MSLS-L), medium (MSLS-M), high (MSLS-H) dose groups. Mice in administration group were given corresponding drugs in advance for 7 days. 1.5 h after the last administration, mice with arrhythmia was established by tail vein injection of 0.4% barium chloride solution. The II-lead electrocardiogram of mice in model group and administration group were monitored by biological signal acquisition system. The expression of Na⁺-K⁺-ATPase and Ca²⁺-Mg²⁺-ATPase in myocardial tissues was detected by ELISA kits. Results Compared with model group, the duration of arrhythmia in GSLS-L, GSLS-M, GSLS-H groups and MSLS-L, MSLS-M, MSLS-H groups were decreased (P<0.01), and there was no statistical difference between GSLS and MSLS at the same dose; In addition, GSLS and MSLS significantly improved the P wave, QRS wave and PQ interval of model mice (P<0.01, P<0.05); The expression of Na⁺-K⁺-ATPase and Ca²⁺-Mg²⁺-ATPase in myocardial tissue of mice in GSLS-M, GSLS-H, MSLS-M and MSLS-H groups were significantly increased (P < 0.01), and there was no statistical difference between GSLS and MSLS at the same dose. Conclusion GSLS and MSLS can improve arrhythmia by increasing the expression of Na⁺-K⁺-ATPase and Ca²⁺-Mg²⁺-ATPase in myocardial tissue, and there was no statistical difference between GSLS and MSLS at the same dose.