黄精多糖对顺铂致肝损害大鼠肝功能的保护及抗氧化指标的影响

目的:观察黄精多糖(Polygonatum sibiricum polysaccharide,PSP)对顺铂(cisplatin,CP)致大鼠肝损害的保护作用及抗氧化指标的影响.方法:采用ip顺铂5 mg· kg-1的方法制备肝损害模型,将40只大鼠随机分为5组:空白组、模型组、PSP低、中、高剂量组(10,20,40 g·kg-1);分别ig处理10 d,采血检测血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)水平;处死后剪取肝测定肝脏系数,肝组织匀浆后检测超氧化物歧化酶(SOD)、丙二醛(MDA)及谷胱甘肽过氧化物酶(GSH-Px)含量;同时观察肝脏组织学结构变化.结果:与空白组比较,模型组大鼠血清ALT,AST含量均显著升高(P＜0.01),肝组织匀浆中SOD,GSH-Px均下降(P＜0.01),而MDA含量升高(P＜0.01).与模型组比较,PSP作用后大鼠血清ALT,AST含量均明显降低(P ＜0.05或P＜0.01),肝组织匀浆中SOD,GSH-Px均明显升高(P＜0.05或P＜0.01),MDA含量明显下降(P＜0.05或P＜0.01).结论:黄精多糖可明显降低顺铂致大鼠肝功能损伤,其机制可能与其抗氧化作用有关.

Protection Effect and Antioxidation of Polygonatum sibiricum Polysaccharide on Liver Function in Cisplatin-induced Hepatotoxicity Rats

Objective: To study the protection effect of Polygonatium sibiricum polysaccharide(PSP) on cisplatin-induced hepatotoxicity. Method: Forty Sprague-Dawley rats were randomly divided into normal control group,model group,the low dosage group,the middle dosage group and the high dosage group of PSP, with eight rats in each group. The model rats with hepatotoxicity were duplicated with injection of cisplatin(5 mg·kg^-1). The rats were injected with cisplatin(5 mg·kg^-1) after fifth days rats in the low, middle and high dosage group of PSP were treated by PSP 10,20,40 g·kg^-1 once a day. After 10 days of administration with PSP, the changes of liver coefficient were examined and the blood alanine aminotrans ferase(ALT), aspartate amino transferasen(AST), the activity of superoxide dismutase(SOD), glutathione peroxidase(GSH-Px) and the content of malondialdehyde(MDA) was measured. Result: Compared with the normal control group, contents of ALT, AST in serum were significantly increased in each treatment group(P<0.01), the content of MDA was significantly increased(P<0.01) and the activity of SOD and GSH-Px was significantly decreased(P<0.01). Compared with the model group, contents of ALT, AST in serum were significantly decreased in each treatment group(P<0.05 or P<0.01), the content of MDA was significantly decreased(P<0.05 or P<0.01) and the activity of SOD and GSH-Px was significantly increased(P<0.05 or P<0.01). Conclusion: PSP may improve the function in liver injury rats induced by cisplatin and inhibite the oxidative damage of liver.