| 酶制剂在玉米秸秆双菌种固态发酵生产微生物油脂中的应用 |
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| 引用本文： | 李新社,陆步诗,金双喜.酶制剂在玉米秸秆双菌种固态发酵生产微生物油脂中的应用[J].中南大学学报(自然科学版),2011,42(6). |
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| 作者姓名： | 李新社 陆步诗 金双喜 |
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| 作者单位： | 邵阳学院生物与化学工程系,湖南邵阳,422000 |
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| 基金项目： | 湖南省科技厅资助科研项目 |
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| 摘 要： | 以玉米秸秆为原料，利用纤维素酶和康宁木霉将玉米秸秆转化为可发酵糖，皮状丝孢酵母利用可发酵糖生产微生物油脂。采用正交试验，分别通过检测发酵物中还原糖含量和油脂产量，确定微生物油脂生产的最佳条件。研究结果表明：利用纤维素酶和康宁木霉处理玉米秸秆，在纤维素酶添加量为0.15％，康宁木霉接种量为10％，处理温度为26℃，处理时间为6 d的条件下，可获得还原糖含量为4.852 g/L的玉米秸秆降解物。利用皮状丝孢和酵母发酵玉米秸秆降解物生产微生物油脂，在皮状丝孢酵母接种量为9％，培养温度为26℃，培养时间为7 d的条件下，每100 g发酵产物中，可获得微生物油脂3.321 g。
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| 关 键 词： | 玉米秸秆 纤维素酶 康宁木霉 皮状丝孢酵母 发酵 微生物油脂 |
Application of enzyme in production of microbial oils fermented by two-strains with corn stalk |
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| LI Xin-she,LU Bu-shi,JIN Shuang-xi.Application of enzyme in production of microbial oils fermented by two-strains with corn stalk[J].Journal of Central South University:Science and Technology,2011,42(6). |
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| Authors: | LI Xin-she LU Bu-shi JIN Shuang-xi |
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| Affiliation: | LI Xin-she,LU Bu-shi,JIN Shuang-xi (Department of Biology and Chemistry Engineering,Shaoyang University,Shaoyang 422000,China) |
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| Abstract: | The corn stalk was treated with cellulase and Trichoderma koningii to produce reducing sugar,then the production was re-fermented by Trichosporon cutaneum to produce microbial oils.The best process conditions were obtained by orthogonal test through measuring the content of reducing sugar and microbial oils in product.The results show that the reducing sugar content of the corn stalk treated with cellulase and Trichoderma koningii is 4.852 g/L under the conditions that the additive of cellulase is 0.15%,and... |
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| Keywords: | corn stalk cellulase Trichoderma koningii Trichosporon cutaneum fermentation microbial oil |
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