TRL型体腔循环灌注热化疗系统安全性评估的动物实验

目的:探讨在不同温度下进行体腔恒温循环热灌注化疗(Coelom Continued Circulatory HyperthermiaPerfusion,CCCHP)对实验动物生命体征和腹腔各脏器的影响,以寻求治疗的最适温度和有效控温的途径。方法:应用TRL型体腔循环灌注热化疗系统建立CCCHP动物模型,选用健康杂种家犬16只分别用41℃(分为单纯生理盐水组和顺铂组),42℃(生理盐水组),43℃(生理盐水组)腹腔温度对每组动物CCCHP 3次(临床治疗一个疗程),每次1h,间隔2d。记录犬的生命体征和治疗的各项参数。在每次CCCHP前及最后一次CCCHP 24h后抽取外周血备检肝肾功。CCCHP3次24h后及2周后时每组分别处死2只犬,开腹观察内脏器官的形态学改变,并切取肝、肾等脏器进行病理检查。结果:41℃腹腔温度下3次CCCHP对犬的生命体征及肝肾功无明显影响,肝、肾、脾和肠组织损伤轻微;41℃腹腔温度联合顺铂3次CCCHP也未对犬的正常生理功能造成明显影响;42℃,43℃腹腔温度行3次CCCHP后均对犬的生命体征及肝肾功有不同程度的影响,肝、肾、脾及肠组织均有不同程度的损伤,损伤程度随温度上升而加重。结论:41℃腹腔温度CCCHP 3次,每次1.0h是安全可行的,可以作为CCCHP联合化疗的安全治疗温度。

Animal Experiment of Security Evaluation of TRL Type Hyperthermic Intraperitoneal Treatment System for Coelom Continued Circulatory Hyperthermia Perfusion

Objective:To investigate the impact of Coelom Continued Circulatory Hyperthermia Perfusion(CCCHP) by TRL type hyperthermic intraperitoneal treatment system with different temperature and treatment conditions on experimental animals vital signs and important organs pathology.Further to search optimal temperature and the way to control intraperitoneal perfusion temperature effectually.Methods:Sixteen medical fitness mongrel dogs were experimented with TRL type hyperthermic intraperitoneal treatment system at 41℃(normal saline group and DDP group),42℃(normal saline group) and 43℃(normal saline group) intraperitoneal temperature.CCCHP was performed three times(one hours per time).During CCCHP,The dogs’ vital signs and instrument parameters were recorded.The animals hepatic and kidney function changes of CCCHP were inspected before the course and after the last CCCHP by preserved blood specime.The general morphological changes and pathological changes of abdominal organs were inspected after the administration 24 hours and 2 weeks.Results:Single CCCHP 41℃ three times has no obvious effects on vital signs and hepatic,renal functions of experiment animals.The liver,kidney,spleen and intestine have only slightly pathological impairments after CCCHP,and which can quickly recover.CCCHP combined with DDP chemotherapy 41℃ three times has no obvious effects on normal physiologic function.CCCHP at 42℃ and 43℃ three times have significant effects to hepatic and renal functions of experiment animals,and liver,kidney,spleen and intestine has significant pathological impairments in different degree after CCCHP.The impairments aggravated with increasing temperature.Conclusion:41℃ intraperitoneal temperature CCCHP three times(one hours per time) is safe and feasible,so it can be choose as conventional thermochemotherapy temperature with CCCHP.