| 鹿茸的HPLC指纹图谱 |
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| 引用本文： | 阎正,于趁,张智慧,蔡立鹏,王春云,苑若瑶,王继坤.鹿茸的HPLC指纹图谱[J].河北大学学报(自然科学版),2010,30(2). |
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| 作者姓名： | 阎正 于趁 张智慧 蔡立鹏 王春云 苑若瑶 王继坤 |
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| 作者单位： | 1. 河北大学化学与环境科学学院,河北,保定,071002
2. 北京中防昊通防伪科技中心,北京,100025 |
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| 基金项目： | 国家科技支撑项目(2006BAK02A17) |
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| 摘 要： | 建立了鹿茸的高效液相指纹图谱.研究了不同的预处理方法、流动相组成、最佳检测波长等操作因素对鹿茸指纹谱图的影响,并对6种不同产地的鹿茸药材的指纹图谱进行了比较.色谱条件为:InertsilODS-3色谱柱(250 mm×4.6 mm;5μm);检测波长270 nm;流动相为甲醇-磷酸水溶液(质量分数为0.05%)梯度洗脱;流速:1 mL/min;分析时间:100 min;柱温为25℃.
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| 关 键 词： | 鹿茸 HPLC 指纹图谱 |
Fingerprint of Pilose Antler by HPLC |
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| YAN Zheng,YU Chen,ZHANG Zhi-hui,CAI Li-peng,WANG Chun-yun,YUAN Ruo-yao,WANG Ji-kun.Fingerprint of Pilose Antler by HPLC[J].Journal of Hebei University (Natural Science Edition),2010,30(2). |
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| Authors: | YAN Zheng YU Chen ZHANG Zhi-hui CAI Li-peng WANG Chun-yun YUAN Ruo-yao WANG Ji-kun |
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| Affiliation: | 1.College of Chemistry and Environmental Science;Hebei University;Baoding 071002;China;2.Zhongfang Haotong Antiforgery Technology Center of Beijing;Beijing 100025;China |
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| Abstract: | This paper described an analytical method of fingerprint of pilose antler by HPLC. different sample pretreatment ,detection wavelength , mobile phase, temperature were studied and the fingerprint chromatograms of pilose antler from six different areas were compared. Inertsil ODS-3 column(250 mm×4.6 mm,5 μm,) was used with the mixture of methanol- phosphoric 0.05% acid;the detection wavelength was 270 nm;the flow rate was 1 mL/min and the column temperature was 25 ℃. |
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| Keywords: | pilose antler HPLC fingerprint |
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