实验性牙髓炎模型兔的构建

背景:以往牙髓炎动物模型的建立大多应用内毒素,不能完全反映临床牙髓炎的形成过程。目的:观察应用变形链球菌诱导兔牙牙髓炎模型不同时间炎症特征。方法:选用20只健康新西兰大白兔,随机将下颌前牙分为实验组和对照组;在兔两颗下颌前牙舌面钻孔,对照组一颗置入生理盐水,实验组另一颗置入变形链球菌,术后1,3,5,7d各处死兔5只;观察牙髓炎炎症转归。结果与结论:实验组术后1d炎症反应较轻,经过3,5,7d牙髓炎症反应逐渐加重,牙髓血管充血、渗出严重,牙髓组织出现坏死。对照组牙髓炎症随着时间的推移,血管扩张逐渐加重,偶有牙髓组织坏死。结果表明,应用变形链球菌诱导牙髓炎症形成过程更符合牙髓炎病理学特征。

Construction of experimental pulpitis models in rabbits

BACKGROUND:Animal models of pulpitis have been established using endotoxin in the past;however,they cannot fully reflect the formation process of clinical pulpitis.OBJECTIVE:To observe the inflammatory characteristics at different time points of rabbit models of pulpitis induced by Streptococcus mutans.METHODS:The mandibular anterior teeth of 20 healthy New Zealand rabbits were divided into experiment group and control group.The lingual surfaces of the rabbit mandibular anterior teeth were drilled;normal saline was imported into the control tooth,while Streptococcus mutans was imported into the experiment tooth.Five rabbits were sacrificed on 1,3,5 and 7 days after operation,respectively;inflammation outcomes of pulpitis was observed.RESULTS AND CONCLUSION:Inflammatory reaction in the experiment group was slight on 1 day after operation,and was aggravated gradually on 3,5 and 7 days after operation,accompanied with vascular hyperemia in the pulp,severe effusion and necrosis of the pulp tissue.Inflammation in the control group was aggravated gradually as time went on;very few necrosis were found in the pulp tissues.These findings indicate that the forming process of pulpitis induced by Streptococcus mutans is more suitable to the pathological features of pulpitis.