金纳米链与人喉癌Hep-2细胞共培养自由进出细胞的形式

背景：金纳米颗粒对肿瘤细胞具有杀伤效应。目的：观察金纳米链对人喉癌Hep-2细胞增殖的影响。方法：首先运用葡萄糖体系合成法制备金纳米链溶胶,然后MTT法检测不同终浓度（10%,25%,50%,75%,95%）金纳米链溶胶对人喉癌Hep-2细胞增殖的影响,并通过电镜观察金纳米链进出Hep-2细胞的过程。结果与结论：金纳米链在75%和95%高浓度时对Hep-2细胞增殖有一定的抑制,但并没有随着浓度的增加而加重,均属于一级范围,表明金纳米链对人喉癌Hep-2细胞无毒性。金纳米链在与Hep-2细胞共培养8h后即能以胞吞的方式进入细胞,48h后大部分出胞,能够自由进出细胞。

Endocytosis and exocytosis of gold nanochain attaching Hep-2 cells of human laryngeal carcinoma

BACKGROUND： The gold nanoparticles have a killing effect on tumor cells. OBJECTIVE： To investigate the influence of gold nanochain on Hep-2 cells proliferation of human laryngeal carcinoma. METHODS： The gold nanochain was prepared by a glucose synthesis method and added into the culture cells with different concentrations （10%, 25%, 50%, 75%, 95%） to test the influence on proliferation of in vitro cultured Hep-2 cells. The endocytosis and exocytosis of transmembrane when gold nanochain attached to Hep-2 cells were observed by electron microscopy. RESULTS AND CONCLUSION： The gold chain at high concentrations （75%, 95%） exhibited inhibitory effects on the proliferation of Hep-2 cells, but the influence was not increased with increasing concentration, belonging to a range of non-toxic. Gold nanchain can enter into Hep-2 cells after 8 hours of co-culture and leave cells after 48 hours, indicating gold nanoparticles chain can enter and leave Hep-2 freely.