非小细胞肺癌中两种雌激素受体亚型与血管内皮生长因子的表达及相关性研究

目的分析非小细胞肺癌（NSCLC）中雌激素受体ER（ERα、ERβ）表达与血管内皮生长因子（VEGF）表达、肿瘤微血管密度（MVD）计数和肿瘤临床指标的相关性,探讨ER表达的意义及其对肺癌微血管生成的影响。方法采用免疫组化EnVision法检测研究54例NSCLC患者,以及10例正常肺组织、10例肺良性肿瘤组织中ERα、ERβ和VEGF的表达及MVD计数。结果正常肺组织和肺良性肿瘤组织中无ERα和ERβ表达,NSCLC中ERα、ERβ和VEGF阳性表达率分别为20.4%（11/54）、45.1%（26/54）和64.8%（35/54）。ERα的表达在各项肺癌临床参数间均无显著性差异;ERβ在腺癌中阳性表达率显著高于鳞癌（P〈0.05）,高分化癌组ERβ阳性表达率显著高于低分化＋中分化癌组（P〈0.05）。VEGF表达在淋巴结转移及肺癌TNM分期间存在差异（P〈0.05）。ERα与VEGF表达、MVD计数呈正相关性（P均〈0.05）。ERβ与VEGF表达、MVD计数无相关性。结论 NSCLC中ERβ表达与病理分类和分化程度有关,提示ERβ表达对NSCLC类型、恶性程度判断具有一定的参考价值。ERα表达与VEGF表达及MVD计数呈正相关性,表明ERα可能通过调节VEGF的表达而促进肺癌微血管的生成。

Correlation and Clinical Significance of Estrogen Receptors and Vascular Endothelial Growth Factor in Non-Small Cell Lung Cancer

Objective To explore the clinical significance of estrogen receptor α （ERα）,estrogen receptor β （ERβ） in non-small cell lung cancer（NSCLC）.Methods EnVision method was used to detect the expressions of ERα,ERβ,vascular endothelial growth factor（VEGF）,and microvessel density（MVD） in 54 NSCLC patients,10 patients with lung benign lesions,and 10 normal controls.The interrelation between ERα,ERβ,VEGF,and MVD was analyzed.Results No obvious expressions of ERα and ERβ were observed in the normal lung tissues and lung benign lesions.The positive expression rates of ERα,ERβ,and VEGF in NSCLC were 20.4%（11/54）,64.8%（35/54）,and 64.8%（35/54）,respectively.There were no significant differences between ERα in regard to clinical parameters of NSCLC.But the expression of ERβ was dependent on pathological classification and differentiation of NSCLC.The expression of ERβ was significantly higher in adenocarcinoma than in squamous cell carcinoma（P0.05）.The expression rate of ERβ in well differentiated group was significantly higher than that in low,moderately differentiated group（P0.05）.There were significant differences between VEGF in regard to lymph node metastasis and TNM stage.The expression of ERα interrelated with VEGF and MVD with r value of 0.4 and 0.685 respectively（P0.05）.There was little correlation between ERβ and VEGF,MVD（P0.05）.Conclusion The expression of ERβ correlates with pathological classification and differentiation of NSCLC,suggesting its significance in evaluating the pathological classification and malignant degree of NSCLC.The expression of ERα correlates with VEGF and MVD,suggesting that ERα possibly promote micro-angiogenesis of NSCLC by VEGF pathway.