蜜环菌诱导子对丹参冠瘿组织积累丹参酮的影响

以 6 7_V液体培养基为基本培养基 ,利用计算机软件“均匀设计、回归分析及优化系统” ,研究了蜜环菌(ArmillariamelleaKarst)诱导子浓度、诱导子的加入时间与处理的收获时间对丹参 (SalviamiltiorrhizaBunge)冠瘿组织积累丹参酮的影响。结果表明 ,蜜环菌诱导子浓度为 119mL/L、第 0天加入诱导子、第 2 9天收获培养物与培养液时可获得最高的丹参酮生产量 (147mg/L ,P <0 .0 5 ) ,蜜环菌诱导子浓度为 113mL/L、第 0天加入诱导子、第 2 6天收获培养液时可获得最高的丹参酮生产量 (6 2mg/L ,P <0 .0 5 ) ,蜜环菌诱导子浓度为 87mL/L、第 0天加入诱导子、第2 8天收获培养物时可获得最高的丹参酮生产量 (94mg/L ,P <0 .0 5 )。结果证实 ,计算机软件“均匀设计、回归分析及优化系统”对于观察值受多因素影响的研究非常有效和方便。

Effect of Armillaria mellea Elicitor on Accumulation of Tanshinones in Crown Gall Cultures of Salvia miltiorrhiza

Using a computer software `uniform design, regression analysis and optimization system' (UROS), the effects of Armillaria mellea Karst elicitor, with respect to its concentration, time of supplement and harvest time, on the accumulation of tanshinones in the crown gall cultures of Salvia miltiorrhiza Bunge in 67_V liquid medium were studied. The result showed that the maximum tanshinone yield was 147 mg/L ( P <0.05), 62 mg/L ( P <0.05), 94 mg/L ( P <0.05) in the liquid medium and the cultures 29 day after addition of A. mellea (119 mL/L) at day 0, in the medium 26 day after the addition of 113 mL/L of A. mellea , 28 day after addition of 87 mL/L of A. mellea , respectively. The results confirmed that the computer software UROS was a good tool in the multi_factor study.