固定化脂肪酶催化（R，JS）-1-苯乙醇转酯化拆分反应及动力学研究

利用制备的舍Fe304无机磁性复合物FSN固定假单胞茵脂肪酶（Pseudomonas sp Lipase，PSL），获得具有高活性、高对映体选择性和易分离的固定化酶PSL／FSN．乙酸乙烯酯做酰化试剂，PSL／FSN在正庚烷溶剂中催化（R，S）-1-苯乙醇转酯化拆分反应，40℃反应2h时，（S）-1-苯乙醇对映体过量值eep和（R）-乙酸-1-苯乙酯的对映体过量值eep均为99％，（R，S）-1-苯乙醇的转化率达到理论值。固定化酶PSL／FSN在70℃经2h热处理，其活性和对映选择性没有明显的下降；PSL／FSN重复使用10次，其活性和对映体选择性未出现衰减。基于实验数据，研究了固定化酶催化（R，S）-1-苯乙醇转酯化拆分反应的动力学行为，获得了（R，S）-1-苯乙醇转酯化拆分反应的动力学方程。

Transesterification Resolution of (R,S)-1-phenylethanol over Immobilized Lipase and Its Kinetic Behavior

The Pseudomonas sp Lipase(PSL) was immobilized on the inorganic magnetic compound support(FSN) containing nanoparticles Fe3O4.The immobilized lipase(PSL/FSN) has higher activity,conversion enantioselectivity and easier recovery advantages comparing with free lipase.PSL/FSN was used in the transesterification resolution of(R,S)-1-phenylethanol.The most suitable acyl donor,organic solvent and reaction temperature was vinyl acetate,respectively,refine n-heptane and 40 ℃.Under these conditions,the ee values of the substrate(S)-1-phenylethanol and the product(R)-1-phenylethyl acetate were both 99% when reaction time is 2 h.After PSL/FSN was incubated 2 hours at 70 ℃,its activity and enantioselectivity was not significantly reduced.The catalytic performance of PSL/FSN was not decreased after PSL/FSN was reused for 10 times.Based on experiment data of transesterification resolution of(R,S)-1-phenylethanol over immobilized lipase reaction,we have investigated its kinetic behavior and got its kinetic equation.