库普弗细胞功能状态对肠缺血再灌注肝、脾、胸腺细胞凋亡的影响

封闭和不封闭ＢＡＬＢ／ｃ小鼠库普弗细胞４８ｈ后，夹闭肠系膜上动脉１ｈ后松夹，复制肠缺血再灌注模型。运用原位细胞ＤＮＡ末端标记和流式细胞术检测缺血前、缺血６０ｍｉｎ、再灌注３０、６０ｍｉｎ肝、脾、胸腺细胞的凋亡情况。

INFLUENCE OF FUNCTIONAL STATE OF KUPFFER CELLS ON APOPTOSIS OF HEPATOCYTES, SPLENOCYTES, AND THYMOCYTES FOLLOWING INTESTINAL ISCHEMIA-REPERFUSION IN MICE

Kupffer cells of BALB/c mice were blocked by injecting GdCl 3 or 0.9% NaCl (7 mg/kg) via tail vein. Forty eight hours later, the superior mesenteric artery was occluded for 60 min followed by declamping to reproduce an intestinal ischemia re perfusion model. TUNEL (TdT mediated dUTP nick end labeling) and flow cytometry were used to detect apoptosis of hepatocytes, splenocytes, and thymocytes. Apoptosis of these cells was increased at 60 min after ischemia, and 30 min and 60 min after reperfusion, and it was even more significantly increased after Kupffer cells blockage with GdCl3.It indicates that Kupffer cells may play an important role in the apoptosis of hepatocytes, splenocytes, and thymocytes following intestinal ischemia repefusion. Kupffer cells might paticipate in modulation of the disturbance of immune system function in multiple organ dysfunction.