| HPV16E7基因沉默对宫颈癌细胞系CaSki细胞增殖的影响 |
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| 引用本文： | 李军果,李 力.HPV16E7基因沉默对宫颈癌细胞系CaSki细胞增殖的影响[J].肿瘤学杂志,2012,18(1):29-33. |
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| 作者姓名： | 李军果 李 力 |
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| 作者单位： | 1. 北京军区总医院生殖医学中心,北京,100700
2. 第三军医大学野战外科研究所,重庆,400042 |
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| 摘 要： | 目的]探讨脂质体转染HPVl6E7siRNA对人宫颈癌CaSki细胞增殖的影响。方法]人工合成抑制HPVl6E7基因的siRNA片段，通过脂质体转染到CaSki细胞内，显微镜下观察其形态学变化；流式细胞术检测各组细胞周期变化；RT-PCR检测HPVl6E7mRNA的表达；Westernblot检测HPVl6E7蛋白表达。结果]转染siRNA后的CaSki细胞，细胞增殖受到显著抑制．HPVl6E7mRNA表达显著下降，HPVl6E7蛋白水平显著下降。结论]应用RNA干扰靶向抑制HPVl6E7基因可以显著抑制CaSki细胞增殖。
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| 关 键 词： | HPVl6E7 siRNA 宫颈肿瘤 |
The Effect of HPV16E7 Gene Silencing on the Proliferation of Cervical Cancer Cell Line CaSki |
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| LI Jun-guo,LI Li.The Effect of HPV16E7 Gene Silencing on the Proliferation of Cervical Cancer Cell Line CaSki[J].Journal of Oncology,2012,18(1):29-33. |
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| Authors: | LI Jun-guo LI Li |
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| Affiliation: | 1.Center of Reproductive Medicine and Genetics,General Hospital of Beijing Army,Beijing 100700,China;2.Research Institute of Surgery,Third Military Medical University,Chongqing 400042,China) |
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| Abstract: | Purpose] To investigate the effect of liposomal transfection of HPV16E7 siRNA on the proliferation of cervical carcinoma cell line CaSki.Methods] HPV16E7 siRNA was synthesized and transfected into CaSki cell by liposome.Cell morphological change was observed with microscopy,and cell proliferation index was detected by flowcytometry.HPV16E7 mRNA expression was determined by RT-PCR and protein level of HPV16E7 was determined by Western blot.Results] For the CaSki cell transfected with siRNA,cell proliferation was inhibited significantly,the expression of HPV16E7 mRNA and protein level of HPV16E7 was significantly decreased.Conclusion] HPV16E7 siRNA can inhibit the proliferation of cervical carcinoma cell line CaSki. |
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| Keywords: | HPV16E7 siRNA cervical neoplasms |
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