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| 华蟾素对耐阿霉素人乳腺癌细胞内阿霉素蓄积的影响 | |
| 引用本文： | 王玲,刘世坤,周于禄,裴奇,阳宁.华蟾素对耐阿霉素人乳腺癌细胞内阿霉素蓄积的影响[J].中国临床药理学与治疗学,2007,12(5):548-551. |
| 作者姓名： | 王玲 刘世坤 周于禄 裴奇 阳宁 |
| 作者单位： | 1. 永州职业技术学院医学院,永州,425006,湖南 2. 中南大学湘雅三医院药剂科,长沙,410006,湖南 3. 永州市人民医院,永州,425006,湖南 |
| 摘 要： | 目的：测定华蟾素（cinobufacine,Cino）对耐阿霉素人乳腺癌细胞MCF-7/ADM内阿霉素（ADM）积累的影响,以探索Cino逆转MCF-7/ADM多药耐药（MDR）的可能机制。方法：MTT法检测CinoMDR的逆转作用,HPLC法检测Cino作用MCF-7/ADM后细胞内ADM的浓度的变化。结果：Cino15mg/L能增加MCF-7/ADM细胞对ADM的敏感性,使ADM的半数抑制浓度（IC50）由38.14mg/L降至12.93mg/L,显著增加MCF-7/ADM株细胞内ADM的浓度。结论：Cino能明显增加MCF-7/ADM株细胞内ADM的含量,部分逆转MCF-7/ADM的MDR。 |
| 关 键 词： | 华蟾素 阿霉素 多药耐药 耐阿霉素人乳腺癌细胞 |
| 文章编号： | 1009-2501（2007）05-0548-04 |
| 修稿时间： | 2006-12-302007-03-30 |
Effects of cinobufacine on intracellular adriamycin accumulation in human breast cancer cells |
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| WANG Ling,LIU Shi-kun,ZHOU Yu-lu,PEI Qi,YANG Ning.Effects of cinobufacine on intracellular adriamycin accumulation in human breast cancer cells[J].Chinese Journal of Clinical Pharmacology and Therapeutics,2007,12(5):548-551. | |
| Authors: | WANG Ling LIU Shi-kun ZHOU Yu-lu PEI Qi YANG Ning |
| Abstract: | AIM: To study the effects of cinobufacine on intracellular adriamycin accumulation in human breast cancer MCF-7/ADM cells, and further explore the mechanism of cinobufacine reversing the multidrug resistance of human breast cancer MCF-7/ADM cells. METHODS: The cytotoxic effect was determined by MTT assay. Intracellular ADM concentration was examined by HPLC. RESULTS: 15 mg/L cinobufacine decreased the IC50 of ADM to MCF-7/ADM cells from 38.14 mg/L to 12.93 mg/L, but increased intracellular adriamycin accumulation in MCF-7/ADM cells. CONCLUSION: Cinobufacine can increase intracellular ADM accumulation and partly reverse MDR of MCF-7/ADM cell line. |
| Keywords: | cinobufacine adriamycin multidrug resistance MCF-7/ADM cell |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
