| 左旋黄皮酰胺在大鼠肝微粒体中的代谢转化研究 |
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| 引用本文： | 姚庆强,王慕邹.左旋黄皮酰胺在大鼠肝微粒体中的代谢转化研究[J].药学学报,1998,33(4):296-299. |
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| 作者姓名： | 姚庆强 王慕邹 |
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| 作者单位： | 中国医学科学院中国协和医科大学药物研究所 |
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| 基金项目： | 卫生部高等院校博士点专项基金 |
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| 摘 要： | 用大鼠肝微粒体体外温孵法进行了左旋黄皮酰胺(-)-clausenamide]代谢转化研究,优化了温孵体系,建立了反相HPLC-DAD同时分离检测左旋黄皮酰胺及其体外代谢产物的分析方法。用硅胶低压柱色谱、制备TLC及制备HPLC分离纯化了两个代谢产物并进行了光谱鉴定。结果表明,两个代谢物分别确定为6-和5-位羟基取代的黄皮酰胺。
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| 关 键 词： | 左旋黄皮酰胺 肝微粒体 高效液相色谱法 |
| 收稿时间： | 1997-07-11 |
Metabolic transformation of (-)-clausenamide in rat liver microsomes] |
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| Yao Qingqiang and Wang Muzou.Metabolic transformation of (-)-clausenamide in rat liver microsomes][J].Acta Pharmaceutica Sinica,1998,33(4):296-299. |
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| Authors: | Yao Qingqiang and Wang Muzou |
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| Affiliation: | Institute of Materia Medica, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100050. |
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| Abstract: | The metabolic transformation of (-)-clausenamide, isolated from the leaves of Clausena lansium (Lour.) Skeels, was studied in vitro with phenobarbital-induced rat liver microsomal incubate containing the NADPH-generating system. The constitution of the incubation system was optimized and a RP-HPLC-DAD method was developed for the on-line analysis of (-)-clausenamide and its metabolites. The major metabolites were isolated and purified by silica gel column chromatography, preparative TLC and HPLC and their structures were determined as 6-OH clausenamide (CM1) and 5-OH clausenamide (CM2) by 1HNMR and MS. |
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| Keywords: | Liver microsomes HPLC (-)-Clausenamide |
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