臭氧对4种细菌的灭活作用

目的:观察不同浓度臭氧在不同作用时间下对金黄色葡萄球菌、变形链球菌、表皮葡萄球菌和肺炎链球菌的灭活作用.方法:用碘滴定法测定打开臭氧发生器不同机组及不同时间时的臭氧浓度,按载体定量杀菌试验,将经臭氧作用的载体与相同环境下未经臭氧作用的载体上的细菌洗下,各取洗脱液50μl,接种于TSA平板并置厌氧箱(90%N2,10%CO2,37°C)培养24～48h后,进行活菌菌落计数.所得数据采用SAS 6.12软件包进行X.检验.结果:臭氧浓度越高、作用时间越长.杀菌效果越好.臭氧浓度2.73mg/L、作用45min时,对4种细菌的杀灭率达100%.结论:臭氧对4种细菌有良好的灭活作用,且杀菌效果有随臭氧浓度增加而增强的趋势.

The disinfecting effect of ozone on four kinds of bacteria

PURPOSE: To evaluate the disinfecting effect of ozone on 4 kinds of bacteria: Staphylococcus aureus, Streptococcus mutans, Staphylococcus epidermidis and Streptococcus pneumoniae. METHODS: The concentration of ozone that was transmitted by ozone generator at different time was determined by using iodine titrimetric method. According to the bactericidal assay of quantitative vehicle, the bacteria on the ozonized vehicles and unozonized vehicles was washed, 50 microl eluant was seeded on the TSA plates. The TSA plates were put into the anaerobiotic incubator (90% N(2),10% CO(2),37 degrees centigrade). After 24 to 48 hours, the CFU on the plate was counted.The data was analyzed by one-way ANOVA with SAS 6.12 software package. RESULTS: The sterilization effect depended on the ozone concentration and the treatment time. When the 4 kinds of bacteria were treated with 2.73 mg/L ozone for 45 minutes, there was no bacteria alive. CONCLUSIONS: Ozone has obvious disinfecting effect on the 4 kinds of bacteria and the effect is correlated with the concentration of ozone.