乳腺癌患者IL-11、CTGF的表达水平与及其与骨转移的相关性

目的分析乳腺癌患者白介素11(IL-11)、结缔组织生长因子(CTGF)的表达水平及其与骨转移的相关性。方法采用酶联免疫吸附试验(ELISA)检测108例乳腺癌患者(乳腺癌组)、30例乳腺良性肿瘤患者(乳腺良性肿瘤组)及健康体检者(对照组)血清中IL-11、CTGF的表达水平,分析其与骨转移的关系,对患者随访2年,比较生存率在不同表达水平之间的差异。结果乳腺癌组血清IL-11、CTGF水平较对照组、乳腺良性肿瘤组均显著升高(P0.05);而乳腺良性肿瘤组与对照组比较,差异均无统计学意义(P0.05)。不同骨转移分级患者的血清IL-11、CTGF水平均显著高于无骨转移患者(P0.05),Ⅲ~Ⅳ级骨转移的乳腺癌患者血清IL-11、CTGF水平较Ⅰ~Ⅱ级患者明显升高(P0.05)。骨转移、无骨转移的乳腺癌患者血清IL-11与CTGF水平均呈正相关(r=0.514、0.477,P0.05)。术后2年,血清IL-11、CTGF高表达患者的生存率明显低于低表达患者(χ2=4.50、5.18,P0.05)。结论乳腺癌患者血清IL-11、CTGF呈过度表达状态,可作为骨转移诊断、预后判断的有效血清学肿瘤标志物。

Expression of IL-11 and CTGF in breast cancer patients and its correlation with bone metastasis

Objective To analyze the expression levels of IL-11 and connective tissue growth factor (CTGF) in patients with breast cancer and their correlation with bone metastasis .Methods The 108 cases of breast cancer(breast cancer group) ,30 cases of breast benign tumor (breast benign tumor group) and 30 cases of healthy controls (control group) were performed by ELISA to de-tect the expression of serum IL-11 ,CTGF ,and their relationship with bone metastases was analyzed .All the patients were followed up for 2 years ,survival rates between different expression levels of IL-11 ,CTGF were compared .Results Compared with those in control group and breast benign tumor group ,the expressions of IL-11 ,CTGF in breast cancer group were increased (P<0 .05) , while there was no significant difference between control group and breast benign tumor group (P<0 .05) .The levels of serum IL-11 ,CTGF in different stages of breast cancer with bone metastases were significantly higher than those in breast cancer without bone metastases(P<0 .05) ,and the levels of IL-11 ,CTGF in bone metastases stage of Ⅲ - Ⅳ were higher than those in bone me-tastases stage ofⅠ - Ⅱ(P<0 .05) .Serum IL-11 and CTGF was positively correlated in breast cancer patients with or without bone metastases(r=0 .514 ,0 .477 ,P<0 .05) .At 2 year after surgery ,the survival rate in patients with high expression of IL-11 and CT-GF was significantly lower than that with low expression (χ2 =4 .50 ,5 .18 ,P<0 .05) .Conclusion The levels of serum IL-11 ,CT-GF in breast cancer patients are overexpressed ,which could be used as an effective serological tumor markers for diagnosis of bone metastases and assessment of prognosis .