| 高效液相色谱-电喷雾电离质谱法分析人参皂甙 |
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| 引用本文： | 王芳,柳翱,李东风.高效液相色谱-电喷雾电离质谱法分析人参皂甙[J].化学工程师,2007,21(4):28-30. |
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| 作者姓名： | 王芳 柳翱 李东风 |
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| 作者单位： | 长春工业大学,生物工程学院,吉林,长春,130012 |
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| 基金项目： | 国家自然科学基金资助项目(20060557) |
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| 摘 要： | 利用反相高效液相色谱-电喷雾电离质谱(HPLC-ESI/MS)方法,分离分析了人参皂甙。液相色谱采用乙腈-水流动相进行梯度洗脱,质谱法利用电喷雾电离质谱(ESI/MS)研究了3种人参皂甙的一级质谱(主要给出分子量)和二级、三级质谱(提供碎片离子信息),并通过质谱图的差异对其结构进行了鉴别,建立了高效液相色谱-质谱联用法分析人参皂甙的方法。
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| 关 键 词： | 高效液相色谱-电喷雾电离质谱法 人参皂甙 人参 |
| 文章编号： | 1002-1124（2007）04-0028-03 |
| 修稿时间： | 2007-01-08 |
Analysis of ginsenosides by HPLC ESJ/MS |
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| WANG Fang,LIU Ao,LI Dong-feng.Analysis of ginsenosides by HPLC ESJ/MS[J].Chemical Engineer,2007,21(4):28-30. |
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| Authors: | WANG Fang LIU Ao LI Dong-feng |
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| Affiliation: | School of Biological Engineering, Changchun University of Technology, Changchun 130012, China |
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| Abstract: | A reversed-phase high performance liquid chromatography-electrospray ionization mass spectrometry method has been developed for the analysis of ginsenosides.A high performance liquid phase chromatography(HPLC) analysis was performed on a revesed-phase C18 colume using mobile phase of acetonitrile and water mixture under gradien elution.HPLC-ESI/MS(gain molecular weight) and HPLC-ESI/MS2 and MS3(fragment ion information)of three ginsenosides has been studied by electrospray ionization mass spectrometry method and it identified the structure of Ginsenosides through the different of mass spectra.Moreover a HPLC-ESI/MS method was established for the analysis of ginsenosides. |
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| Keywords: | high performance liquid chromatography-electrospray ionization mass spectrometry ginsenosides panax ginseng |
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