玄参多糖对2型糖尿病大鼠糖脂代谢及肝胰岛素信号通路的影响

目的探究玄参多糖对2型糖尿病大鼠糖脂代谢及肝胰岛素信号通路的影响。方法采用链脲佐菌素(STZ)注射法制备糖尿病大鼠模型,随机分成5组,分别为模型组、阳性药二甲双胍(200 mg/kg)组及玄参多糖低、中、高剂量(80、160、320 mg/kg)组,另设同周龄普通饲料喂养大鼠为对照组;ig给药6周后监测各组大鼠体质量及生存状态、空腹血糖(FBG)、血脂总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)]、肝功能丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)]、肾功能肌酐(CREA)、血尿素氮(BUN)]、糖化血红蛋白(GHb)、空腹血清胰岛素(FINS)、C-肽、超氧化物歧化酶(SOD)、丙二醛(MDA)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)等指标的变化;HE染色和油红O染色评估大鼠肝脏病理学变化及脂肪变性;Western blotting法检测肝胰岛素信号通路相关蛋白表达。结果玄参多糖能回升2型糖尿病大鼠体质量,改善代谢功能,降低大鼠FBG、GHb、ALT、AST、CREA、BUN、TC、TG、LDL-C、MDA水平,升高HDL-C、FINS、C-肽、SOD、CAT、GSH-Px水平;Western blotting结果表明玄参多糖能活化IRS-2/PI3K/Akt信号通路,升高过氧化物酶体增殖物激活受体γ(PPAR-γ)、葡萄糖转运蛋白4(GLUT-4)表达水平。结论玄参多糖能够改善2型糖尿病大鼠糖脂代谢,其机制可能跟调控肝胰岛素信号通路有关。

Effects of polysaccharides from Scrophulariae Radix on glucolipid metabolism and hepatic insulin signaling pathway in type 2 diabetic rats

Objective To explore effects of polysaccharides from Scrophulariae Radix(PFR) on glucolipid metabolism and hepatic insulin signaling pathway in type 2 diabetic rats. Methods Diabetic rat models were constructed by STZ injection, and randomly divided into five groups: model group, low, medium and high dose groups(80, 160, 320 mg/kg) of PFR, and positive drug group of metformin(200 mg/kg);In addition, ordinary feed rats of the same age were set as normal group;Body weight and survival status, fasting blood glucose(FBG), blood lipid(TC, TG, HDL-C, LDL-C), liver function(ALT, AST), renal function(CREA, BUN), glycosylated hemoglobin(GHb), insulin secretion(FINS), C-peptide, SOD, MDA, CAT, GSH-Px, and other indicators were detected after drug delivery for six weeks;HE staining and oil red O staining were used to evaluate the pathological changes and steatosis of liver in rats;Western blotting was used to detect the changes of hepatic insulin signaling pathway related protein. Results PFR increased the weight of diabetic rats, improved the metabolic function, reducde the content of FBG, GHb, ALT, AST, CREA, BUN, TC, TG, LDL-C, MDA and increased the content of HDL-C, FINS, C-peptide, SOD, CAT and GSH-Px;Western blotting assay showed that PFR could activate IRS-2/PI3 K/Akt signaling pathway and increase the protein levels of PPAR-γ and GLUT-4. Conclusion PFR could improve glucolipid metabolism in type 2 diabetic rats by regulating the hepatic insulin signaling pathway.