斑马鱼饲养循环装置中的水生环境微生物群落代谢

目的在斑马鱼标准化养殖中，建立饲养环境中微生物群落代谢指纹数据库，用于分析和判断饲养环境是否出现病原菌感染。方法在不当的饲养过程中，斑马鱼会出现死亡或疾病，其中一个重要原因是病原菌感染。采用MicroStation细菌鉴定系统，微生物群落将鉴定板上的95种碳源作为唯一可利用碳源，当各孔中微生物利用碳源底物时，呼吸作用引起了氧化还原反应指示剂颜色变化，板上出现的颜色反应作为鉴定的指纹。结果多变量分析代谢的类型与程度，得出健康的斑马鱼饲养环境的微生物群落对GN和GP板上的22种有共同利用的模式。结论本文通过微生物群落代谢单碳源方法分析微生物群落的结构和功能变化，多变量分析后定位健康的斑马鱼饲养环境中的微生物群落代谢特征。

Application of Community-Level Physiological Profiles （CLPP） to Evaluate Aquatic Environment of Laboratory Zebrafish Raising in Circulating Aquariums

Objective To set up microbial community-level physiological fingerprint profiling database in four different aquariums feeding zebrafish, analyze and assess whether micropathogens infect zebrafish during standard raising. Methods MicroStation microbial identification system was used. Microbial community utilized 95 compounds for carbon source on identification microplate. When microbial pathogens utilized carbon substances in a well, respiration lead change of oxidation and deoxidized indicator, and color change becomes identification fingerprint. Result In this experiment, 22 carbon sources on GN and GP microplates were utilized by all of healthy microbial community in zebrafish aquariums based on multivariable analysis. Conclusion This approach, called community-level physiological profiling （CLPP）, has been effective for distinguishing healthy and pathogenic microbial communities in the aquariums, and provided a set of healthy fingerprint database.