异种脱细胞角膜基质囊袋移植的生物相容性研究

目的探讨脱细胞猪角膜基质移植入兔角膜囊袋后的生物学反应。方法猪角膜通过不同方式去除细胞及免疫源性成分，保留角膜组织基质的弹力纤维及胶原纤维，将其切取为直径为4mm的植片，植入兔角膜囊袋内，在不同时间点观察生物材料在角膜内生物学反应。结果材料植入兔角膜囊袋3个月，生物相容性良好，材料逐渐降解，材料内有胶原和角膜基质细胞长人。结论新型可降解角膜基质材料植入后未见兔角膜有明显的炎症反应，材料的组织相容性好，可作为组织工程角膜的支架材料。

Study on biocompatibility of acellular cornea stroma xenograft in stromal pocket

Objective To study the biocompatibility of acellular cornea stroma implanted in rabbit cornea stroma. Methods The cornea stroma was obtained from adult hog,cells and immunogenic component were removed through different extraction methods, and the elastic and collagen fibers were preserved. Stromal disc with 4 mm diameter was implanted into interlamellar stromal pocket and followed up until 3 months. The immunoreaction of stromal disc was observed with HE staining at different time points. Results The advers immunoreaction was not found in the stromal lamella during experiment and follow up. After implantation in 2 weeks,acellular cornea stroma become transparent ;after implantation in 3 months,the rabbit stroma cells immigrated into the xenograft,the acellular cornea stromal were gradually degraded. Conclusion It demonstrates that the acellular cornea stroma processes excellent histocompatiblility ,thus it is a good materials for constructing tissue-engineered cornea.