基因枪在肿瘤基因治疗中的应用价值

目的 研究基因枪经小鼠皮肤转移基因的效率、被转基因的表达量、表达时间，以及对小鼠肿瘤的治疗效果，探讨作为一种 基因转移工具，基因枪在基因治疗中应用的可能性。方法 （１）用荧光显微镜观察基因枪对体外培养细胞系的基因转导效率和被转基因表达时间；（２）通过基因枪向小鼠皮肤转Ｌａｃ￣Ｚ基因后，取转瓣皮肤做冰浇灌发片进行β－ｇａｌ染色，观察转基因蛋白在皮人中表达的部位；（３）用ＥＬＩＳＡ法检测向小鼠皮肤转ｍ

The use of gene gun in cancer gene therapy

OBJECTIVE: With Helios gene gun, the report genes EGFP and Lac-Z were transfected to several cultured mammalian tumor cell lines in vitro and mice skin in vivo, respectively. A stable long time exogene's expression were got. Then, the pWRG3142, which carried mGM-CSF gene was delivered to the abdominal skin of C57BL/6 mice using gene gun. Pathological sections showed the local transproteins' expression companying with a profound inflammation reaction characterized by neutrophilic infiltration. ELISA assay of transfected mouse's serum sample indicated a high improvement of transgenic proteins level, which demonstrated that transgenic GM-CSF secreted from treated skin into the bloodstream effectively. In B16 melanoma tumor model, mice immunized with mGM-CSF expression plasmids could be partially protected from 1 x 10(5) B16 cells challenge and exhibited a drastically reduced tumor growth rate. Finally, we conclucled that exogenes could be transfected into cultured cell lines and mice skin effectively by gene gun technology, and gene gun mediated in vivo delivery of GM-CSF cDNA should be further developed for potential clinical testing as an approach for human cancer gene therapy.