Plasmid-encoded ropiness production inLactobacillus casei SSP.casei |
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Authors: | Marisa Vescovo G L Scolari V Bottazzi |
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Affiliation: | (1) Istituto di Microbiologia, Università Cattolica, 29100 Piacenza, Italy |
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Abstract: | Summary Genetic determinants of the Muc+ character were investigated in two ropy strains,Lactobacillus delbrueckii ssp.bulgaricus 201 andL. casei ssp.casei NCIB 4114, which secrete a large amount of slime in culture media. Plasmid DNA analysis revealed the presence of two plasmids (4.5 and 2.3 Mdal) inL. casei ssp.casei, whileL. delbrueckii ssp.bulgaricus was plasmid free, suggesting a chromosomal location of Muc+ character in this strain. Curing experiments carried out onL. casei ssp.casei NCIB 4114 indicated a correlation between the Muc+ phenotype and the 4.5 Mdal plasmid. |
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