| SHP-1对人乳腺癌MCF-7细胞侵袭的影响及机制初步探讨 |
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| 引用本文： | 黄维甄,邹爱梅,石敏,罗荣城,李荣.SHP-1对人乳腺癌MCF-7细胞侵袭的影响及机制初步探讨[J].热带医学杂志,2011,11(3):253-256,361. |
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| 作者姓名： | 黄维甄 邹爱梅 石敏 罗荣城 李荣 |
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| 作者单位： | 南方医科大学南方医院肿瘤中心,广东广州,510515 |
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| 摘 要： | 目的探讨SHP-1对人乳腺癌细胞株MCF-7侵袭能力的影响。方法 MCF-7细胞株分别瞬时转染SHP-1的特异性siRNA干扰序列;然后分别通过RT-PCR和Western-blot检测MCF-7细胞干扰前后SHP-1基因和蛋白表达变化,并通过RT-PCR检测干扰前后MMP-9基因变化;通过Transwell小室实验检测干扰SHP-1对细胞侵袭能力的影响。结果 RT-PCR和Western-blot证实SHP-1在人乳腺癌细胞株MCF-7中有表达以及SHP-1SiRNA的干扰效率;干扰SHP-1后,MMP-9的表达量上调;Transwell小室实验证实,与MCF-7阴性干扰对照及空白对照细胞相比,siRNA组的侵袭能力显著增强(P<0.001)。结论乳腺癌MCF-7中SHP-1的表达水平高低和该细胞侵袭能力高低呈负相关,其侵袭可能和MMP-9的表达呈正相关。
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| 关 键 词： | SHP-1基因 乳腺癌 侵袭 MCF-7细胞 |
Effects of SHP-1 on the invasion of MCF-7 breast cancer cells and the preliminary mechanism |
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| HUANG Wei-zhen,ZOU Ai-mei,SHI Min,LUO Rong-cheng,LI Rong.Effects of SHP-1 on the invasion of MCF-7 breast cancer cells and the preliminary mechanism[J].Journal Of Tropical Medicine,2011,11(3):253-256,361. |
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| Authors: | HUANG Wei-zhen ZOU Ai-mei SHI Min LUO Rong-cheng LI Rong |
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| Affiliation: | HUANG Wei-zhen,ZOU Ai-mei,SHI Min,LUO Rong-cheng,LI Rong(Department of Oncology,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China) |
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| Abstract: | Objective To investigate the effects of SH2 domain-containing cytosolic protein tyrosine phosphatase(SHP-1) on the invasion of MCF-7 breast cancer cells.Methods Cells were transiently transfected with siRNA of the SHP-1 mRNA,then they were analyzed for the presence of SHP-1 using Real-time PCR and western blot analysis.The expression of MMP-9 was detected by Real-time PCR.Cells were analyzed for cell invasion ability using transwell chamber invasion assay.Results SHP-1 gene and protein were found in MCF-7 c... |
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| Keywords: | SHP-1 gene breast cancer invasion MCF-7 cells |
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