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Circular dichroism detection in high-performance liquid chromatography: Evaluation of the anisotropy factor |
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| Authors: | Carlo Bertucci Enrico Domenici and Piero Salvadori |
| Affiliation: | Centro di Studio del C.N.R. per le Macromolecole Stereordinate ed Otticamente Attive, Dipartimento di Chimica e Chimica Industriale, Universitá di Pisa, via Risorgimento, 35, 56126 Pisa, Italy |
| Abstract: | The application of a circular dichroism (c.d.) detection system in HPLC using a chiral stationary phase is presented. The simultaneous measurement of the absorbance and c.d. signal allows the evaluation of the anisotropy factor (g = Δ /) and thus the determination of the enantiomeric excess (e.e.) of the eluates. When this detection system is used in preparative chiral chromatography the collection of the enantiomeric fractions can be readily optimized. |
| Keywords: | Circular dichroism liquid chromatography detection system chiral discrimination drug analysis stereochemistry Pirkle phase g-factor enantiomeric excess preparative LC |
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