糯米藤多糖的提取工艺优化

目的:研究提取糯米藤多糖的最佳工艺条件。方法:以多糖提取率为考察指标,溶剂回流作为提取手段,利用单因素及正交试验确定影响提取的主要因素,然后使用回归正交组合设计优化提取工艺。结果:影响糯米藤多糖提取率的因素顺序为提取温度>液固比>提取时间>提取次数>浸泡时间;回流提取的最佳工艺条件为在96.26℃水浴条件下,提取时间109.20min、提取次数2次、液固比27.28:1(mL/g)、浸泡时间90min,最优工艺条件下多糖提取率可达25.121%。结论:使用回归正交设计能合理地优化糯米藤多糖的提取工艺。

Optimization of Extraction Process for Polysccharides from Whole Plants of Gonostegia hirta (BL.) Miq.

Objective: To find optimal conditions for the extraction of polysaccharides from whole plants of Gonostegia hirta(BL.) Miq.Methods: Solvent reflux extraction was used for polysaccharide extraction.Five parameters that influence extraction efficiency were investigated using one-factor-at-a-time and orthogonal array design methods,and three main ones were optimized based on a quadratic rotation-orthogonal composite experimental design.Results: In decreasing order of their effect on extraction efficiency,five influential parameters were ranked as extraction temperature > solvent-to-solid ratio > extraction time >extraction number>soaking time.Their optimal levels were 96.26 ℃,27.28:1(mL/g),109.20 min each time,2 and 90 min,respectively,resulting in an extraction yield of 25.121%.Conclusion: Quadratic rotation-orthogonal composite experimental design permits reasonable optimization of polysaccharide extraction from Gonostegia hirta(BL.) Miq.