纤维素酶催化与三液相萃取偶联制备盾叶薯蓣皂苷元

利用三液相萃取与酶催化转化耦合技术对盾叶薯蓣中薯蓣皂苷进行水解，并分离提取薯蓣皂苷元。考察了几种甾体皂苷和水解下的葡萄糖在三液相中的分配、酶的分配以及酶活力在三液相体系中的保留情况，并对比了三液相、有机相、水相中纤维素酶催化转化与薯蓣皂苷元的生成情况。实验结果表明，正己烷/1,4-二氧六环/硫酸铵三液相体系能够满足酶催化和产物分离同步进行的要求，使薯蓣皂苷元分配在正己烷相，底物和酶保留在1,4-二氧六环相，大部分葡萄糖进入硫酸铵相，反应进行96h薯蓣皂苷元的得率为69.4%，比有机相、水相酶催化分别提高一倍和27.6倍，显示出耦合技术的明显优势。

Preparation of diosgenin by integrated bioprocess of cellulase catalysis and extration in three-liquid-phase system

Using coupling biotech of cellulase-catalysis and extrusion in three-liquid-phase system that composed of n-hexane,1,4-dioxane and ammonium sulfate to hydrolyze dioscin in Chinese yan and glucose connected to steroidal saponins,and extration separation to collect diosgenin.The distribution of several steroidal saponins,hydrolyzed glucose,enzyme and its activity in the three-phase system were studied,and catalytic conversion by cellulase-enzyme and formation of diosgenin in three-liquid,organic and aqueous phases were compared.The results showed that diosgenin yield in the three-liquid-phase system could compare with that in organic phase and aqueous phase.The three-liquid-phase system could be suitable for simultaneous enzymatic hydrolysis of steroidal saponins and extration of diosgenin and glucose.The substrate and enzyme were held in the middle phase of 1,4-dioxane,whiles the products,diosgenin and glucose,were partitioned into the top and bottom phase,respectively.The yields of diosgenin was 69.4% at 96 h in the three-liquid-phase system,which was twice and 27.6 folds obtained in organic phase and aqueous phase respectively.