| 鹿流行性出血病病毒双抗夹心ELISA方法的建立 |
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| 引用本文： | 陈兵,李健波,杨俊兴,阮周曦,孙洁,张彩虹,刘建利,花群义,周晓黎.鹿流行性出血病病毒双抗夹心ELISA方法的建立[J].动物医学进展,2011(10):1-5. |
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| 作者姓名： | 陈兵 李健波 杨俊兴 阮周曦 孙洁 张彩虹 刘建利 花群义 周晓黎 |
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| 作者单位： | 深圳出入境检验检疫局;云南出入境检验检疫局; |
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| 基金项目： | 国家863计划项目(2006AA10Z445); 中国博士后科学基金(20080430855) |
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| 摘 要： | 为建立鹿流行性出血病病毒(EHDV)病原学检测方法,用纯化的抗EHDV特异性单克隆抗体包被ELISA板,用兔抗EHDV IgG作为夹心抗体,IgG作为夹心抗体建立EHDV双抗夹心ELISA方法,并对该方法的特异性和敏感性进行了试验.用ELISA分别检测EHDV、蓝舌病病毒(BTV)、水疱性口炎病毒(VSV)、赤羽病病毒...
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| 关 键 词： | 鹿流行性出病 鹿流行性出病病毒 双抗体夹心ELISA 单克隆抗体 |
Establishment of Sandwich ELISA for Epizootic Hemorrhagic Disease of Deer Virus |
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| CHEN Bing,LI Jian-bo,YANG Jun-xing,RUAN Zhou-xi,SUN Jie,ZHANG Cai-hong,LIU Jian-li,HUA Qun-yi,ZHOU Xiao-li.Establishment of Sandwich ELISA for Epizootic Hemorrhagic Disease of Deer Virus[J].Progress In Veterinary Medicine,2011(10):1-5. |
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| Authors: | CHEN Bing LI Jian-bo YANG Jun-xing RUAN Zhou-xi SUN Jie ZHANG Cai-hong LIU Jian-li HUA Qun-yi ZHOU Xiao-li |
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| Affiliation: | CHEN Bing1,LI Jian-bo1,YANG Jun-xing1,RUAN Zhou-xi1,SUN Jie1,ZHANG Cai-hong1,LIU Jian-li1,HUA Qun-yi1,ZHOU Xiao-li2(1.Shenzhen Entry-Exit Inspection and Quarantine Bureau,Shengzhen,Guangdong,518001,China,2.Yunnan Entry-Exit Inspection and Quarantine Bureau,Kunming,Yunnan,650228,China) |
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| Abstract: | In order to establish an immunoassay for Epizootic hemorrhagic disease of deer virus(EHDV) detection,a double-antibody sandwich ELISA was developed based on purified monoclonal antibody against EHDV and rabbit anti EHDV polyclonal antibody.This ELISA was used to detect positive samples of EHDV,bluetongue virus(BTV),Vesicular stomatitis virus(VSV),Akabane disease virus(AKV) and Peste des petits ruminants virus(PPRV) and negative samples,and the results indicated that the ELISA had good specificity.This ELISA... |
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| Keywords: | Epizootic hemorrhagic disease Epizootic hemorrhagic disease of deer virus sandwich ELISA monoclonal antibody |
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