Electron immunolocalization of type IV collagen, laminin and fibronectin synthesized by multilayered cells cultured from human oral epithelium |
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| Authors: | H Poly M L Couble D J Hartmann M Faure H Magloire |
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| Affiliation: | Laboratoire d'Histophysiologie et de Pathologie des Tissus Dentaires, Facultéd'Odontologie de Lyon, Hopital E. Herriot, Lyon. France;Centre de Radioanalyse, Institut Pasteur de Lyon, Hopital E. Herriot, Lyon. France;UnitéINSERM de Recherches Dermalologiques et d'lmmunologie, Hopital E. Herriot, Lyon. France |
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| Abstract: | Human palatal epithelial cells were grown on lethally irradiated 3T3 fibroblasts. Under these conditions, epithelial cultures become stratified. The basal cells have a cuboïdal shape and suprabasal layers are flattened. The reconstituted epithelium exhibits markers of differentiation: intracytoplasmic bundles of keratin-like filaments and abundant desmosmes present in every layer. Type IV collagen fibronectin and laminin are synthesized by these cultures. Basal cells grip the culture substratum via an extracellular matrix made up of type IV collagen, laminin and fibronectin. |
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