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| 产纤维素酶菌群的筛选及其酶学特性研究 | |
| 引用本文： | 宋安东,王 磊,仉 磊,章晓庆,杜 然,李十中.产纤维素酶菌群的筛选及其酶学特性研究[J].中国生态农业学报,2010,18(2):356. |
| 作者姓名： | 宋安东 王 磊 仉 磊 章晓庆 杜 然 李十中 |
| 作者单位： | 1. 河南农业大学生命科学学院,郑州,450002 2. 河南农业大学生命科学学院,郑州,450002;清华大学核能与新能源技术研究院,北京,100084 3. 清华大学核能与新能源技术研究院,北京,100084 |
| 基金项目： | 国家"十一五"科技支撑计划项目(2006BAD07A01)资助 |
| 摘 要： | 从常年堆放的腐质豆秆中分离到1组产纤维素酶菌群MO,并在50℃、pH8.0条件下培养,90h时达到最高酶活,活性为1.3611IU。该酶最适反应温度为60℃,pH为7.0,在60℃以下和pH3~8范围内具有良好的热稳定性和pH稳定性。在最适反应条件下,该酶的最高活性可达2.13IU。通过生长动力学研究了菌群内部不同生长阶段pH、酶活和失重率的相互关系,并通过非变性电泳对酶谱进行初步研究,得到7条活性条带,说明MO中具有多种产酶菌株。 |
| 关 键 词： | 纤维素酶 菌群 CMCase活性 非变性电泳 菌群动态变化 |
| 收稿时间： | 2009/3/13 0:00:00 |
| 修稿时间： | 6/9/2009 12:00:00 AM |
Isolation and enzymatic characteristics of cellulase producing microbial community |
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| SONG An-Dong,WANG Lei,ZHANG Lei,ZHANG Xiao-Qing,DU Ran and LI Shi-Zhong.Isolation and enzymatic characteristics of cellulase producing microbial community[J].Chinese Journal of Eco-Agriculture,2010,18(2):356. | |
| Authors: | SONG An-Dong WANG Lei ZHANG Lei ZHANG Xiao-Qing DU Ran and LI Shi-Zhong |
| Affiliation: | College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China;1. College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China 2. Institute of Nuclear and New Energy Technology, Tsinghna University, Beijing 100084, China;Institute of Nuclear and New Energy Technology, Tsinghna University, Beijing 100084, China;Institute of Nuclear and New Energy Technology, Tsinghna University, Beijing 100084, China;Institute of Nuclear and New Energy Technology, Tsinghna University, Beijing 100084, China;Institute of Nuclear and New Energy Technology, Tsinghna University, Beijing 100084, China |
| Abstract: | A cellulase producing microbial community (MO) was isolated from rotted beanstalk compost. When the community was cultured at pH 8.0 and 50 ℃, maximal CMCase activity (1.361 1 IU) is observed in 90 hours. The optimal conditions for enzymatic reaction are 60 ℃ at pH 7.0. High heat and pH stability is observed at temperature lower than 60 ℃ and pH 3～8. CMCase activity of cellulase can reach 2.13 IU at optimal reaction point. Growth kinetics indicate some relationships among pH, CMCase activity and weight loss rate in different growth phases of microbial community. Through cellulase analysis for zymogram via native-PAGE, seven activity bands are observed. This suggests that more than one strain is responsible for the production of enzyme in MO. |
| Keywords: | Cellulase Microbial community CMCase activity Native-PAGE Dynamic variation |
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