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花椰菜游离小孢子培养研究进展
引用本文:李念祖,汤青林,王志敏,等.花椰菜游离小孢子培养研究进展[J].长江蔬菜,2011(8):4-6.
作者姓名:李念祖  汤青林  王志敏  
作者单位:1. 西南大学园艺园林学院
2. 南方山地园艺学教育部重点实验室
3. 重庆市蔬菜学重点实验室,重庆,400715
基金项目:国家自然科学基金,中央高校基本科研业务费专项资金,重庆市自然科学基金,西南大学博士基金
摘    要:分析了影响花椰菜游离小孢子培养的主要因素(材料基因型、小孢子发育时期、预处理和预培养、培养基成分、培养技术),并对胚状体发生与植株再生、DH纯系获得等的研究情况进行了阐述,展望了游离小孢子培养技术发展前景.

关 键 词:花椰菜  游离小孢子  培养条件

Research on Dissociated Microspore's Culture of Brassica oleracea var.botrytis
LI Nianzu,TANG Qinglin,WANG Zhimin,SONG Ming.Research on Dissociated Microspore's Culture of Brassica oleracea var.botrytis[J].Journal of Changjiang Vegetables,2011(8):4-6.
Authors:LI Nianzu  TANG Qinglin  WANG Zhimin  SONG Ming
Affiliation:(College of Horticulture and Landscape Architecture,Southwest University/Key Laboratory of Horticulture Science for Southern Mountainous Regions,Ministry of Education/Key Laboratory of Olericulture,Chongqing 400715)
Abstract:This article summarized the main factors which affect the dissociated microspore’s culture of Brassica oleracea var.botrytis(the genotype of the material,the growth period of the dissociated microspores,pretreatment and pre-cultivation,the consisting of the culture,the technology),the embryoid’s occurrence,the regeneration of the plant,and also the acquirement of DH lines are related.The foreground of the dissociated microspore’s culture was also prospected.
Keywords:Brassica oleracea var  botrytis  Dissociated microspore  Culture condition
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